Search results for APC/Fire

APC/Fire™ 810 anti-mouse CD45 Antibody, CD45, 30-F11, BioLegend, 103174

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

APC/Fire™ 810 anti-mouse CD197 (CCR7) Antibody, CD197, 4B12, BioLegend, 120135

The 4B12 antibody does not inhibit binding of ligand to receptor. Additional reported applications (for the relevant formats) include: immunoprecipitation. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302) is recommended prior to immunofluorescent staining.Staining with clone 4B12 is recommended at 37°C(see supplemental data of PE staining at differing temperatures).

APC/Fire™ 810 anti-mouse CD197 (CCR7) Antibody, CD197, 4B12, BioLegend, 120136

The 4B12 antibody does not inhibit binding of ligand to receptor. Additional reported applications (for the relevant formats) include: immunoprecipitation. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302) is recommended prior to immunofluorescent staining.Staining with clone 4B12 is recommended at 37°C(see supplemental data of PE staining at differing temperatures).

APC/Fire 750 anti-human CD36 Antibody anti-CD36 - 5-271, BioLegend, 336219

Additional reported applications (for the relevant formats) include: immunofluorescence2.

APC/Fire 750 anti-human CD36 Antibody anti-CD36 - 5-271, BioLegend, 336220

Additional reported applications (for the relevant formats) include: immunofluorescence2.

APC/Fire™ 810 anti-human CD56 (NCAM) Antibody, CD56, 5.1H11, BioLegend, 362577

APC/Fire™ 810 anti-human CD56 (NCAM) Antibody, CD56, 5.1H11, BioLegend, 362578

APC/Fire 750 anti-mouse CD8a Antibody anti-CD8a - 53-6.7, BioLegend, 100765

Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3,in vivoandin vitrocell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, immunohistochemical staining5,6of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).

APC/Fire 750 anti-mouse CD8a Antibody anti-CD8a - 53-6.7, BioLegend, 100766

Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3,in vivoandin vitrocell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, immunohistochemical staining5,6of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).

APC/Fire™ 810 anti-mouse CD8a Antibody, CD8a, 53-6.7, BioLegend, 104007

Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3,in vivoandin vitrocell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, immunohistochemical staining5,6of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).