Search results for Anti-Neurofilament H
Alexa Fluor 488 anti-Neurofilament H NF-H, Nonphosphorylated Antibody anti-Neurofilament - SMI 32, BioLegend, 801705
Additional reported applications (for the relevant formats) include Western blotting6, immunohistochemistry4,5, immunocytochemistry1,2,3, 7, array tomography8.Cross-reactivity to monkey tissue has been Reported in the literature, not verified in house4.This antibody reacts with a nonphosphorylated epitope in neurofilament H of most mammalian species. The reaction is masked when the epitope is phosphorylated. The staining of isolated neurofilament preparations is greatly intensified upon dephosphorylation. Immunocytochemically, SMI 32 visualizes neuronal cell bodies, dendrites, and some thick axons in the central and peripheral nervous systems. However, thin axons are not revealed. Other cells and tissues are unreactive. The antibody distinguishes three subdivisions of the macaque precentral motor cortex. The greater size of the left versus the right superior temporal lobe was found to be due to increased axonal myelination and not due to increased number of glial cells or SMI 32-enumerated neurons, suggesting that the specialization for language in the left temporal lobe is related to increased speed of signal transmission. In cultures of murine cortex, SMI 32 labels a neuronal population with enhanced vulnerability to kainate toxicity most of which are GABAergic and reveal kainate-activated Ca2+uptake.
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC-P -Quality tested
Conjugation
Alexa Fluor 488 anti-Neurofilament H NF-H, Nonphosphorylated Antibody anti-Neurofilament - SMI 32, BioLegend, 801706
Additional reported applications (for the relevant formats) include Western blotting6, immunohistochemistry4,5, immunocytochemistry1,2,3, 7, array tomography8.Cross-reactivity to monkey tissue has been Reported in the literature, not verified in house4.This antibody reacts with a nonphosphorylated epitope in neurofilament H of most mammalian species. The reaction is masked when the epitope is phosphorylated. The staining of isolated neurofilament preparations is greatly intensified upon dephosphorylation. Immunocytochemically, SMI 32 visualizes neuronal cell bodies, dendrites, and some thick axons in the central and peripheral nervous systems. However, thin axons are not revealed. Other cells and tissues are unreactive. The antibody distinguishes three subdivisions of the macaque precentral motor cortex. The greater size of the left versus the right superior temporal lobe was found to be due to increased axonal myelination and not due to increased number of glial cells or SMI 32-enumerated neurons, suggesting that the specialization for language in the left temporal lobe is related to increased speed of signal transmission. In cultures of murine cortex, SMI 32 labels a neuronal population with enhanced vulnerability to kainate toxicity most of which are GABAergic and reveal kainate-activated Ca2+uptake.
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC-P -Quality tested
Conjugation
Purified anti-Neurofilament H & M (NF-H/NF-M), Non-Phosphorylated Antibody - SMI 33, BioLegend, 835403
Cell bodies and dendrites are generally unstained. Other cells and tissues are unreactive except for peripheral axons.
Host
Mouse
Reactivity
Rat, Mouse, Human
Applications
IHC-P -Quality testedWB -Verified
Conjugation
Purified anti-Neurofilament H & M (NF-H/NF-M), Non-Phosphorylated Antibody - SMI 33, BioLegend, 835404
Cell bodies and dendrites are generally unstained. Other cells and tissues are unreactive except for peripheral axons.
Host
Mouse
Reactivity
Rat, Mouse, Human
Applications
IHC-P -Quality testedWB -Verified
Conjugation
Alexa Fluor 594 anti-Neurofilament H & M NF-H/NF-M, Hypophosphorylated - SMI-35, BioLegend, 835609
Additional reported applications (for the relevant formats of this clone): include ELISA Capture1-3, immunohistochemical staining on frozen tissue sections, immunofluorescence staining, and spatial biology (IBEX)6,7.Clone SMI 35 reacts with highly phosphorylated neurofilaments, as well as with low degrees of phosphorylation. It primarily reacts with neurofilament H and with neurofilament M to a lesser extent.Notes: On two dimensional gels, this antibody detects a band extending from the phosphorylated neurofilament position at 200 kD (pI 5.1) toward the non-phosphorylated position at 170 kD (pI 6.2).
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC-P - Quality testedICC - Verified
Conjugation
Alexa Fluor 594 anti-Neurofilament H & M NF-H/NF-M, Hypophosphorylated - SMI-35, BioLegend, 835610
Additional reported applications (for the relevant formats of this clone): include ELISA Capture1-3, immunohistochemical staining on frozen tissue sections, immunofluorescence staining, and spatial biology (IBEX)6,7.Clone SMI 35 reacts with highly phosphorylated neurofilaments, as well as with low degrees of phosphorylation. It primarily reacts with neurofilament H and with neurofilament M to a lesser extent.Notes: On two dimensional gels, this antibody detects a band extending from the phosphorylated neurofilament position at 200 kD (pI 5.1) toward the non-phosphorylated position at 170 kD (pI 6.2).
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC-P - Quality testedICC - Verified
Conjugation
Alexa Fluor 647 anti-Neurofilament H & M NF-H/NF-M, Phosphorylated Antibody - SMI 310, BioLegend, 837709
Additional reported applications (for the relevant formats) include: Western blotting4, immunocytochemistry2, immunohistochemical staining of frozen tissue sections1,3, and spatial biology (IBEX)5,6.Clone SMI 310 reacts with an extensively phosphorylated epitope of neurofilament H and, to a much lesser extent, neurofilament M in most mammalian species. Phosphatase treatment of samples abolishes reaction with SMI 310. A very extensive degree of hyperphosphorylation of neurofilaments seems to be necessary for its reactivity.
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC-P -Quality testedSB -Reported in the literature, not verified in house
Conjugation
Alexa Fluor 647 anti-Neurofilament H & M NF-H/NF-M, Phosphorylated Antibody - SMI 310, BioLegend, 837710
Additional reported applications (for the relevant formats) include: Western blotting4, immunocytochemistry2, immunohistochemical staining of frozen tissue sections1,3, and spatial biology (IBEX)5,6.Clone SMI 310 reacts with an extensively phosphorylated epitope of neurofilament H and, to a much lesser extent, neurofilament M in most mammalian species. Phosphatase treatment of samples abolishes reaction with SMI 310. A very extensive degree of hyperphosphorylation of neurofilaments seems to be necessary for its reactivity.
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC-P -Quality testedSB -Reported in the literature, not verified in house
Conjugation
Spark YG™ 570 anti-Neurofilament H (NF-H), Nonphosphorylated Antibody, Neurofilament H, SMI 32, BioLegend, 801713
Additional reported applications (for the relevant formats) include Western blotting6, immunohistochemistry4,5, immunocytochemistry1,2,3, 7, array tomography8.Cross-reactivity to monkey tissue has been Reported in the literature, not verified in house4.This antibody reacts with a nonphosphorylated epitope in neurofilament H of most mammalian species. The reaction is masked when the epitope is phosphorylated. The staining of isolated neurofilament preparations is greatly intensified upon dephosphorylation. Immunocytochemically, SMI 32 visualizes neuronal cell bodies, dendrites, and some thick axons in the central and peripheral nervous systems. However, thin axons are not revealed. Other cells and tissues are unreactive. The antibody distinguishes three subdivisions of the macaque precentral motor cortex. The greater size of the left versus the right superior temporal lobe was found to be due to increased axonal myelination and not due to increased number of glial cells or SMI 32-enumerated neurons, suggesting that the specialization for language in the left temporal lobe is related to increased speed of signal transmission. In cultures of murine cortex, SMI 32 labels a neuronal population with enhanced vulnerability to kainate toxicity most of which are GABAergic and reveal kainate-activated Ca2+uptake.
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC-P - Quality testedIHC-F - Verified
Conjugation
Spark YG™ 570 anti-Neurofilament H (NF-H), Nonphosphorylated Antibody, Neurofilament H, SMI 32, BioLegend, 801714
Additional reported applications (for the relevant formats) include Western blotting6, immunohistochemistry4,5, immunocytochemistry1,2,3, 7, array tomography8.Cross-reactivity to monkey tissue has been Reported in the literature, not verified in house4.This antibody reacts with a nonphosphorylated epitope in neurofilament H of most mammalian species. The reaction is masked when the epitope is phosphorylated. The staining of isolated neurofilament preparations is greatly intensified upon dephosphorylation. Immunocytochemically, SMI 32 visualizes neuronal cell bodies, dendrites, and some thick axons in the central and peripheral nervous systems. However, thin axons are not revealed. Other cells and tissues are unreactive. The antibody distinguishes three subdivisions of the macaque precentral motor cortex. The greater size of the left versus the right superior temporal lobe was found to be due to increased axonal myelination and not due to increased number of glial cells or SMI 32-enumerated neurons, suggesting that the specialization for language in the left temporal lobe is related to increased speed of signal transmission. In cultures of murine cortex, SMI 32 labels a neuronal population with enhanced vulnerability to kainate toxicity most of which are GABAergic and reveal kainate-activated Ca2+uptake.
Host
Mouse
Reactivity
Human, Mouse, Rat
Applications
IHC-P - Quality testedIHC-F - Verified
Conjugation
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