Search results for Lck antibody
Purified anti-Lck Antibody anti-Lck - A17013D, BioLegend, 601002
Clone A17013D was derived from a human Lck peptide phosphorylated at Y505 immunogen, but still displayed pan reactivity towards the unmodified peptide during western blot screening. This clone was moderately stronger than BioLegend clone LCK-01 for western blot and substantially stronger for immunofluorescence microscopy in parallel testing. But it shows a weak affinity towards mouse Lck. This clone is predicted to recognize both 58 and 61 kD isoforms based off the presence of the immunizing epitope in both proteins and may also recognize certain SRC tyrosine kinase family members due to high sequence similarity.Phosphorylation of Lck Y505 reduces binding of this antibody by western blot (please see Fig. 3); we recommend LCK-01 as a pan control antibody.The additional band of ~ 50 kD detected bellow the canonical Lck band in IP experiment could be Lck degradation product.
Host
Mouse
Reactivity
Human, Mouse
Applications
WB -Quality testedICC, IP -Verified
Conjugation
Proto-Oncogene Tyrosine-Protein Kinase LCK (LCK) Antibody, Abbexa, abx113550
Lymphocyte-Specific Protein Tyrosine Kinase Antibody is a Rabbit Polyclonal antibody against Lymphocyte-Specific Protein Tyrosine Kinase.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Applications
ELISA, WB, IHC
Conjugation
Unconjugated
![Alexa Fluor® 750 Anti-Lck antibody [Y123] Brand Logo](https://file.buyantibody.com/public/brand/20260608/Abcam.jpg "Click to View Alexa Fluor® 750 Anti-Lck antibody [Y123] Product Detail")
Alexa Fluor® 750 Anti-Lck antibody [Y123], Abcam, AB321584
Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . How are conjugated primary antibodies validated? This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling. For suitable applications and species reactivity, please refer to the unconjugated version of this clone. Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
Host
Rabbit
Reactivity
Human
Applications
Target Binding Affinity, Flow Cyt (Intra), ICC/IF, Antibody Labelling, IHC-P
Conjugation
Alexa Fluor® 750
Proto-Oncogene Tyrosine-Protein Kinase LCK (LCK) Antibody, Abbexa, abx328129
LCK Antibody is a Rabbit Polyclonal against LCK.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Applications
ELISA, WB, IF/ICC
Conjugation
Unconjugated
Alexa Fluor® 488 Anti-Lck antibody [Y123], Abcam, AB320621
Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . How are conjugated primary antibodies validated? This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling. For suitable applications and species reactivity, please refer to the unconjugated version of this clone. Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
Host
Rabbit
Reactivity
Human
Applications
Target Binding Affinity, Antibody Labelling, ICC/IF, IHC-P, Flow Cyt (Intra)
Conjugation
Alexa Fluor® 488
Proto-Oncogene Tyrosine-Protein Kinase LCK (LCK) Antibody, Abbexa, abx329603
LCK Antibody is a Rabbit Polyclonal against LCK.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Applications
ELISA, WB, IHC, IF/ICC
Conjugation
Unconjugated
Proto-Oncogene Tyrosine-Protein Kinase LCK (LCK) Antibody, Abbexa, abx031732
LCK is a member of the Src family of protein tyrosine kinases (PTKs). This protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules.
Host
Rabbit
Reactivity
Human
Applications
ELISA, WB, IHC, FCM
Conjugation
Unconjugated
Proto-Oncogene Tyrosine-Protein Kinase LCK (LCK) Antibody, Abbexa, abx012318
Tyrosine kinase that plays an essential role for the selection and maturation of developing t-cell in the thymus and in mature t-cell function. is constitutively associated with the cytoplasmic portions of the cd4 and cd8 surface receptors and plays a key role in t-cell antigen receptor (tcr)-linked signal transduction pathways. association of the tcr with a peptide antigen-bound mhc complex facilitates the interaction of cd4 and cd8 with mhc class ii and class i molecules, respectively, and thereby recruits the associated lck to the vicinity of the tcr/cd3 complex. lck then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (itams) in the cytoplasmic tails of the tcr gamma chains and cd3 subunits, initiating the tcr/cd3 signaling pathway. in addition, contributes to signaling by other receptor molecules. associates directly with the cytoplasmic tail of cd2, and upon engagement of the cd2 molecule, lck undergoes hyperphosphorylation and activation. also plays a role in the il2 receptor-linked signaling pathway that controls t-cell proliferative response. binding of il2 to its receptor results in increased activity of lck. is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-tcr and mature alpha beta tcr.
Host
Mouse
Reactivity
Human
Applications
ELISA, WB
Conjugation
Unconjugated
Proto-Oncogene Tyrosine-Protein Kinase LCK (LCK) Antibody, Abbexa, abx234726
LCK Antibody is a Rabbit Polyclonal against LCK.
Host
Rabbit
Reactivity
Human, Mouse
Applications
ELISA, WB
Conjugation
Unconjugated
Proto-Oncogene Tyrosine-Protein Kinase LCK (LCK) Antibody, Abbexa, abx234727
LCK Antibody is a Mouse Monoclonal against LCK.
Host
Mouse
Reactivity
Human, rat
Applications
ELISA, WB
Conjugation
Unconjugated
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