Search results for 605
Brilliant Violet 605 anti-mouse/human CD45R/B220 Antibody anti-CD45R - RA3-6B2, BioLegend, 103244
Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1,in vitroandin vivomodulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.
Host
Rat
Reactivity
Mouse, Human
Applications
FC - Quality tested
Conjugation
Brilliant Violet 605™ anti-human CD206 (MMR) Antibody anti-CD206 - 15-2, BioLegend, 321139
The 15-2 antibody blocks the interaction of MMR with its ligand, and inhibits mannose receptor-mediated degradation of t-PA by macrophages. Additional reported applications of this antibody (for the relevant formats) include: Western blotting1, blocking of ligand binding1,2, immunofluorescence3, and immunohistochemical staining of acetone-fixed frozen tissue sections1. The Utra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 321149 and 321150).
Host
Mouse
Reactivity
Human
Applications
FC -Quality tested
Conjugation
Brilliant Violet 605™ anti-human CD206 (MMR) Antibody anti-CD206 - 15-2, BioLegend, 321140
The 15-2 antibody blocks the interaction of MMR with its ligand, and inhibits mannose receptor-mediated degradation of t-PA by macrophages. Additional reported applications of this antibody (for the relevant formats) include: Western blotting1, blocking of ligand binding1,2, immunofluorescence3, and immunohistochemical staining of acetone-fixed frozen tissue sections1. The Utra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 321149 and 321150).
Host
Mouse
Reactivity
Human
Applications
FC -Quality tested
Conjugation
Brilliant Violet 605 anti-mouse IL-10 Antibody anti-IL-10 - JES5-16E3, BioLegend, 505031
ELISA or ELISPOT Detection1,9,11:The biotinylated JES5-16E3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified JES5-2A5 antibody (Cat. Nos. 504902 & 504904) as the capture antibody.ELISA Capture: The purified JES5-16E3 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated JES5-2A5 antibody (Cat. Nos. 504905 & 504906) as the detection antibody and recombinant mouse IL-10 (Cat. No. 575809) as the standard.Neutralization14:The Ultra-LEAF™ purified JES5-16E3 antibody can neutralize the bioactivity of natural or recombinant IL-10.Flow Cytometry3: The fluorochrome-labeled JES5-16E3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-10-producing cells within mixed cell populations.Additional reported applications (for relevant formats) include:immunohistochemistry3.
Host
Rat
Reactivity
Mouse
Applications
ICFC - Quality tested
Conjugation
Brilliant Violet 605 anti-mouse IL-2 Antibody anti-IL-2 - JES6-5H4, BioLegend, 503829
ELISA Detection1-3or ELISPOT Detection4-6: The biotinylated JES6-5H4 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified JES6-1A12 antibody (Cat. Nos. 503701 & 503702) as capture antibody and recombinant mouse IL-2 (Cat. No. 575409) as the standard.Flow Cytometry8-10: The fluorochrome-labeled JES6-5H4 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-2 -producing cells within mixed cell populations.Neutralization1,7: The Ultra-LEAF™ purified antibody (Endotoxinin vivoandin vitro(Cat. No. 503845-503850)) is recommended for neutralization.Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections2,in vivocapture7, and immunocytochemistry.Note:For testing mouse IL-2 in serum, plasma or supernatant, BioLegend's ELISA MAX™ Sets (Cat. No. 431001 & 431004) are specially developed and recommended.
Host
Rat
Reactivity
Mouse
Applications
ICFC - Quality tested
Conjugation
Brilliant Violet 605 anti-human Ki-67 Antibody anti-Ki-67 - Ki-67, BioLegend, 350521
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1, Western blotting3, and immunofluorescence microscopy4.Ki-67 Staining Protocol:1. Prepare 70% ethanol and chill at -20°C.2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xgfor 5 minutes.3. Discard supernatant and loosen the cell pellet by vortexing.4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 106/ml.7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.
Host
Mouse
Reactivity
Human
Applications
ICFC - Quality tested
Conjugation
Brilliant Violet 605 anti-human Ki-67 Antibody anti-Ki-67 - Ki-67, BioLegend, 350522
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1, Western blotting3, and immunofluorescence microscopy4.Ki-67 Staining Protocol:1. Prepare 70% ethanol and chill at -20°C.2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xgfor 5 minutes.3. Discard supernatant and loosen the cell pellet by vortexing.4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 106/ml.7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.
Host
Mouse
Reactivity
Human
Applications
ICFC - Quality tested
Conjugation
Brilliant Violet 605 anti-mouse CD134 OX-40 Antibody anti-CD134 - OX-86, BioLegend, 119419
Clone OX-86 has been reported to act as an agonist and stimulate OX-40.
Host
Rat
Reactivity
Mouse
Applications
FC -Quality tested
Conjugation
Brilliant Violet 605™ anti-mouse/rat/human CD27 Antibody, CD27, LG.3A10, BioLegend, 124249
Additional reported applications (for the relevant formats) include: functional assay1,3and immunohistomecial staining of acetone-fixed frozen sections2. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 124243 & 124244).
Host
Armenian Hamster
Reactivity
Mouse, Rat, Human
Applications
FC -Quality tested
Conjugation
Brilliant Violet 605™ anti-human CD152 CTLA-4 Antibody anti-CD152 - BNI3, BioLegend, 369609
Based on in-house testing, we do not recommend using clone BNI3 for immunohistochemistry of paraffin-embedded tissue section.
Host
Mouse
Reactivity
Human
Applications
ICFC -Quality testedFC -Verified
Conjugation
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