Search results for 605

Brilliant Violet 605™ anti-human/mouse Granzyme B Recombinant Antibody, Granzyme B, QA18A28, BioLegend, 396433

Clone QA18A28 is compatible with Fixation buffer (Cat. No. 420801) and Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002) in both C57BL/6 splenocytes and human PBMC. QA18A28 is also compatible with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401).

Brilliant Violet 605™ anti-human/mouse Granzyme B Recombinant Antibody, Granzyme B, QA18A28, BioLegend, 396434

Clone QA18A28 is compatible with Fixation buffer (Cat. No. 420801) and Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002) in both C57BL/6 splenocytes and human PBMC. QA18A28 is also compatible with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401).

Brilliant Violet 605 anti-human TCR α/β Antibody anti-TCR α/β - IP26, BioLegend, 306731

Additional reported applications (for the relevant formats) include: T cell activation. When co-staining with anti-CD3, we recommend using clone UCHT1, since we have confirmed that IP26 does not compete with this clone. Other anti-CD3 clones may compete out the binding of IP26.

Brilliant Violet 605 anti-human TCR α/β Antibody anti-TCR α/β - IP26, BioLegend, 306732

Additional reported applications (for the relevant formats) include: T cell activation. When co-staining with anti-CD3, we recommend using clone UCHT1, since we have confirmed that IP26 does not compete with this clone. Other anti-CD3 clones may compete out the binding of IP26.

Brilliant Violet 605 anti-human-mouse-rat CD278 ICOS Antibody anti-CD278 - C398-4A, BioLegend, 313537

The C398.4A antibody is useful for flow cytometric analysis and is able to costimulate T cell activation and proliferation. Additional reported applications (for the relevant formats) include: immunoprecipitation1,in vitrocostimulation of T cell activation1,3,4, and spatial biology (IBEX)5,6. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 313512).

Brilliant Violet 605 anti-human-mouse-rat CD278 ICOS Antibody anti-CD278 - C398-4A, BioLegend, 313538

The C398.4A antibody is useful for flow cytometric analysis and is able to costimulate T cell activation and proliferation. Additional reported applications (for the relevant formats) include: immunoprecipitation1,in vitrocostimulation of T cell activation1,3,4, and spatial biology (IBEX)5,6. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 313512).

Brilliant Violet 605™ anti-mouse IL-33Rα (IL1RL1, ST2) Antibody, IL-33Rα, DIH9, BioLegend, 145323

Brilliant Violet 605 anti-human CD274 B7-H1, PD-L1 Antibody anti-CD274 - 29E.2A3, BioLegend, 329723

Clone 29E.2A3 is reported to recognize an epitope on PD-L1 within the PD-L1-CD80 binding region5. Additional reported applications (for the relevant formats) include: blocking1-3and immunohistochemical staining of acetone-fixed frozen sections1. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329715, 329716, 329745 - 329748).It has been observed that clone 29E.2A3 is able to bind to Alexa Fluor® 700 antibody conjugates during multi-color immunofluorescent staining. This interaction can be resolved by sequentially staining with the 29E.2A3 antibody first and then followed by the Alexa Fluor® 700 conjugate of interest.Clone 29E.2A3 does not work in Western blot applications7.

Brilliant Violet 605 anti-human CD274 B7-H1, PD-L1 Antibody anti-CD274 - 29E.2A3, BioLegend, 329724

Clone 29E.2A3 is reported to recognize an epitope on PD-L1 within the PD-L1-CD80 binding region5. Additional reported applications (for the relevant formats) include: blocking1-3and immunohistochemical staining of acetone-fixed frozen sections1. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329715, 329716, 329745 - 329748).It has been observed that clone 29E.2A3 is able to bind to Alexa Fluor® 700 antibody conjugates during multi-color immunofluorescent staining. This interaction can be resolved by sequentially staining with the 29E.2A3 antibody first and then followed by the Alexa Fluor® 700 conjugate of interest.Clone 29E.2A3 does not work in Western blot applications7.

Brilliant Violet 605 anti-human HLA-A,B,C Antibody anti-HLA-A,B,C - W6/32, BioLegend, 311431

Clone W6/32 recognizes residues in the N terminus of the human ß2-microglobulin molecule21.Additional reported applications (for the relevant formats) include: immunoprecipitaton2, Western blotting (non-reducing)3, immunohistochemical staining of acetone-fixed frozen tissue sections4,5, blocking6,7, inhibition of NK cell-mediated lysis10, and activation8,9. Clone W6/32 has been reported not to be suitable for immunohistochemistry on paraffin sections17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 311428) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).