Search results for 53
APC/Fire™ 750 anti-mouse CD8b.2 Antibody, CD8b.2, 53-5.8, BioLegend, 140420
Additional reported applications (for the relevant formats) include: immunofluorescence3, immunohistochemical staining4of frozen tissue section using dry ice-isopentane method and immunoprecipitation5.
Host
Rat
Reactivity
Mouse
Applications
FC -Quality tested
Conjugation
.png "Click to View Phospho-ATF-2 (Thr69/71)/ATF-7 (Thr51/53) (E6A8A) Rabbit Monoclonal Antibody#61584 Product Detail")
Phospho-ATF-2 (Thr69/71)/ATF-7 (Thr51/53) (E6A8A) Rabbit Monoclonal Antibody#61584, Cell Signaling Technology (CST), 61584
Phospho-ATF-2 (Thr69/71)/ATF-7 (Thr51/53) (E6A8A) Rabbit Monoclonal Antibody detects endogenous levels of ATF-2 only when dually phosphorylated at both Thr69 and Thr71, and ATF-7 only when dually phosphorylated at both Thr51 and Thr53. This antibody does not recognize ATF-2 singly phosphorylated at either Thr69 or Thr71, and it does not recognize ATF-7 singly phosphorylated at either Thr51 or Thr53.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Applications
Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:1600 - 1:6400 Flow Cytometry (Fixed/Permeabilized): 1:800 - 1:3200
Conjugation
Spark Red™ 718 anti-mouse CD8a (Flexi-Fluor™) Antibody, CD8a, 53-6.7, BioLegend, 285050
Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3,in vivoandin vitrocell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, immunohistochemical staining5,6of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).
Host
Rat
Reactivity
Mouse
Applications
FC
Conjugation
Spark YG™ 581 anti-mouse CD8a (Flexi-Fluor™) Antibody, CD8a, 53-6.7, BioLegend, 285380
Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3,in vivoandin vitrocell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, immunohistochemical staining5,6of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).
Host
Rat
Reactivity
Mouse
Applications
FC
Conjugation
Spark YG™ 593 anti-mouse CD8a (Flexi-Fluor™) Antibody, CD8a, 53-6.7, BioLegend, 285424
Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3,in vivoandin vitrocell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, immunohistochemical staining5,6of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).
Host
Rat
Reactivity
Mouse
Applications
FC
Conjugation
Phospho-ATF-2 (Thr69/71)/ATF-7 (Thr51/53) (E6A8A) Rabbit Monoclonal Antibody (BSA and Azide Free)#94125, Cell Signaling Technology (CST), 94125
Phospho-ATF-2 (Thr69/71)/ATF-7 (Thr51/53) (E6A8A) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of ATF-2 only when dually phosphorylated at both Thr69 and Thr71, and ATF-7 only when dually phosphorylated at both Thr51 and Thr53. This antibody does not recognize ATF-2 singly phosphorylated at either Thr69 or Thr71, and this antibody does not recognize ATF-7 singly phosphorylated at either Thr51 or Thr53.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Applications
Conjugation
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