Search results for 387
Spark UV™ 387 anti-human CD45RA Antibody, CD45RA, HI100, BioLegend, 304180
Additional reported applications (for relevant formats of this clone) include: inhibition of CD45 functions2, immunohistochemical staining of frozen tissue sections3and formalin-fixed paraffin-embedded tissue sections4, and immunocytochemistry15,16.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Spark UV™ 387 anti-human CD45 Antibody, CD45, HI30, BioLegend, 304085
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections9, inhibition of CD45 functions4, immunofluorescence11, Western blotting3, and spatial biology (IBEX)16,17.It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Spark UV™ 387 anti-human CD45 Antibody, CD45, HI30, BioLegend, 304086
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections9, inhibition of CD45 functions4, immunofluorescence11, Western blotting3, and spatial biology (IBEX)16,17.It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Spark UV™ 387 anti-human CD19 Antibody, CD19, HIB19, BioLegend, 302289
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections8and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302267 & 302268).Clone HIB19 partially blocks anti-human CD19 clones 4G7 and SJ25C1 staining based on in-house testing
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Spark UV™ 387 anti-human CD19 Antibody, CD19, HIB19, BioLegend, 302290
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections8and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302267 & 302268).Clone HIB19 partially blocks anti-human CD19 clones 4G7 and SJ25C1 staining based on in-house testing
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Spark UV™ 387 anti-human CD3 Antibody, CD3, HIT3a, BioLegend, 300337
Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and activation of T lymphocytes4-7. The HIT3a antibody is able to stimulate T cell activation. The LEAF™purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300314). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300332) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Spark UV™ 387 anti-human CD3 Antibody, CD3, HIT3a, BioLegend, 300338
Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and activation of T lymphocytes4-7. The HIT3a antibody is able to stimulate T cell activation. The LEAF™purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300314). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300332) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Spark UV™ 387 anti-human CD11b Antibody, CD11b, ICRF44, BioLegend, 301365
The ICRF44 antibody inhibits heterotypic adhesion of granulocytes in response to fMLP. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, immunofluorescence microscopy5, stimulation of monocytes3, blocking of heterotypic PMN aggregation8, and blocking of granulocyte activation12. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 301361 & 301362).
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Applications
FC - Quality tested
Conjugation
Spark UV™ 387 anti-human CD11b Antibody, CD11b, ICRF44, BioLegend, 301366
The ICRF44 antibody inhibits heterotypic adhesion of granulocytes in response to fMLP. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, immunofluorescence microscopy5, stimulation of monocytes3, blocking of heterotypic PMN aggregation8, and blocking of granulocyte activation12. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 301361 & 301362).
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Applications
FC - Quality tested
Conjugation
Spark UV™ 387 anti-mouse CD11c Antibody, CD11c, N418, BioLegend, 117369
Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, immunofluorescence microscopy5, 9(Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10), and spatial biology (IBEX)22,23.
Host
Armenian Hamster
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
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