Search results for 515

Spark Blue™ 515 anti-human IgM Antibody, IgM, MHM-88, BioLegend, 314557
MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation

Spark Blue™ 515 anti-human IgM Antibody, IgM, MHM-88, BioLegend, 314558
MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation

Spark Blue™ 515 anti-mouse CD45 Antibody, CD45, 30-F11, BioLegend, 103185
Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation

Spark Blue™ 515 anti-mouse CD45 Antibody, CD45, 30-F11, BioLegend, 103186
Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation

Spark Blue™ 515 anti-mouse CD8a Antibody, CD8a, 53-6.7, BioLegend, 104005
Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3,in vivoandin vitrocell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, immunohistochemical staining5,6of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation

Spark Blue™ 515 anti-mouse CD8a Antibody, CD8a, 53-6.7, BioLegend, 104006
Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3,in vivoandin vitrocell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, immunohistochemical staining5,6of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation

Spark Blue™ 515 anti-human CD57 Antibody, CD57, HNK-1, BioLegend, 359627
Additional reported applications for the relevant formats include: Western blotting1.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation

Spark Blue™ 515 anti-human CD57 Antibody, CD57, HNK-1, BioLegend, 359628
Additional reported applications for the relevant formats include: Western blotting1.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation

Spark Blue™ 515 anti-human CD141 (Thrombomodulin) Antibody, CD141, M80, BioLegend, 344137
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation

Spark Blue™ 515 anti-human CD141 (Thrombomodulin) Antibody, CD141, M80, BioLegend, 344138
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
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