Search results for 550
Spark Blue™ 550 anti-mouse CD4 Antibody, CD4, GK1.5, BioLegend, 100474
Additional reported applications (for the relevant formats) include: blocking of CD4+T cell activation1,4,11, thymocyte costimulation3,in vitroandin vivodepletion2,5-8, blocking of egg-sperm cell adhesion1,4, immunohistochemical staining of acetone-fixed frozen sections9,10, immunoprecipitation1,2, and spatial biology (IBEX)12,13. The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
Host
Rat
Reactivity
Mouse
Applications
FC -Quality tested
Conjugation
Spark Blue™ 550 anti-human CD45RA Antibody, CD45RA, HI100, BioLegend, 304185
Additional reported applications (for relevant formats of this clone) include: inhibition of CD45 functions2, immunohistochemical staining of frozen tissue sections3and formalin-fixed paraffin-embedded tissue sections4, and immunocytochemistry15,16.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Spark Blue™ 550 anti-human CD45RA Antibody, CD45RA, HI100, BioLegend, 304186
Additional reported applications (for relevant formats of this clone) include: inhibition of CD45 functions2, immunohistochemical staining of frozen tissue sections3and formalin-fixed paraffin-embedded tissue sections4, and immunocytochemistry15,16.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Spark Blue™ 550 anti-mouse CD11c Antibody, CD11c, N418, BioLegend, 117365
Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, immunofluorescence microscopy5, 9(Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10), and spatial biology (IBEX)22,23.
Host
Armenian Hamster
Reactivity
Mouse
Applications
FC -Quality tested
Conjugation
Spark Blue™ 550 anti-mouse CD11c Antibody, CD11c, N418, BioLegend, 117366
Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, immunofluorescence microscopy5, 9(Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10), and spatial biology (IBEX)22,23.
Host
Armenian Hamster
Reactivity
Mouse
Applications
FC -Quality tested
Conjugation
Spark Blue™ 550 anti-human CD8 Antibody, CD8a, SK1, BioLegend, 344759
Clone SK1 recognizes the a chain of CD8. Additional reported applications (for the relevant formats) include: proteogenomics8, immunohistochemistry of acetone-fixed frozen tissue sections, and spatial biology (IBEX)9,10. This clone was tested in-house and does not demonstrate utility for formalin-fixed paraffin-embedded (FFPE) human tonsil sections.
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Applications
FC -Quality tested
Conjugation
Spark Blue™ 550 anti-human CD8 Antibody, CD8a, SK1, BioLegend, 344760
Clone SK1 recognizes the a chain of CD8. Additional reported applications (for the relevant formats) include: proteogenomics8, immunohistochemistry of acetone-fixed frozen tissue sections, and spatial biology (IBEX)9,10. This clone was tested in-house and does not demonstrate utility for formalin-fixed paraffin-embedded (FFPE) human tonsil sections.
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Applications
FC -Quality tested
Conjugation
Spark Blue™ 550 anti-human CD3 Antibody, CD3, SK7, BioLegend, 344851
Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections4,5,8, immunofluorescent staining6, and Western blotting3.
Host
Mouse
Reactivity
Human
Applications
FC -Quality tested
Conjugation
Spark Blue™ 550 anti-human CD3 Antibody, CD3, SK7, BioLegend, 344852
Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections4,5,8, immunofluorescent staining6, and Western blotting3.
Host
Mouse
Reactivity
Human
Applications
FC -Quality tested
Conjugation
![Anti-COX2 / Cyclooxygenase 2 antibody [EPR25651-550] - BSA and Azide free (Capture) Brand Logo](https://file.buyantibody.com/public/brand/20260608/Abcam.jpg "Click to View Anti-COX2 / Cyclooxygenase 2 antibody [EPR25651-550] - BSA and Azide free (Capture) Product Detail")
Anti-COX2 / Cyclooxygenase 2 antibody [EPR25651-550] - BSA and Azide free (Capture), Abcam, AB323358
ab323358 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human COX2 / Cyclooxygenase 2. The recommended pair for sandwich ELISA is: Capture: ab323358, Human COX2 / Cyclooxygenase 2 Capture Antibody (unconjugated) Detector: ab323359 , Human COX2 / Cyclooxygenase 2 Detector Antibody (unconjugated) The reference range value is 0.04-10 ng/mL. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Sandwich ELISA The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Host
Rabbit
Reactivity
Human
Applications
sELISA
Conjugation
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