Search results for IL-6 Antibody
Biotin anti-rat IL-6 Antibody anti-IL-6 - Poly5177, BioLegend, 517703
Poly5177 is 4.1% cross-reactive with mouse IL-6 as measured by ELISA.
Host
Goat
Reactivity
Rat
Applications
ELISA Detection - Quality tested
Conjugation
![Anti-IL-6 antibody [IL-6-10] - BSA and Azide free (Capture) Brand Logo](https://file.buyantibody.com/public/brand/20260608/Abcam.jpg "Click to View Anti-IL-6 antibody [IL-6-10] - BSA and Azide free (Capture) Product Detail")
Anti-IL-6 antibody [IL-6-10] - BSA and Azide free (Capture), Abcam, AB244758
ab244758 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human/Monkey IL-6. The recommended pair for sandwich ELISA is: Capture: ab244758, Human/Monkey IL-6 Capture Antibody (unconjugated) Detector: ab244886 , Human/Monkey IL-6 Detector Antibody (unconjugated) The reference range value is 7.8 - 500 pg/ml for human and 12.5 - 800 pg/ml for monkey. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Sandwich ELISA The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Host
Rabbit
Reactivity
Human, Monkey
Applications
sELISA
Conjugation
Anti-IL-6 antibody [IL-6-5] - BSA and Azide free (Detector), Abcam, AB244886
What is this antibody validated in? Anti-IL-6 antibody [IL-6-5] - BSA and Azide free (Detector) (ab244886) is a rabbit recombinant monoclonal antibody and is validated for use in ELISA in Human, Monkey samples. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Sandwich ELISA The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Host
Rabbit
Reactivity
Human, Monkey
Applications
sELISA
Conjugation
Purified anti-mouse IL-6 Antibody anti-IL-6 - MP5-20F3, BioLegend, 504501
ELISA Capture1-3,11or ELISPOT Capture4,5:The purified MP5-20F3 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP5-32C11 antibody (Cat. No. 504602) as the detecting antibody and recombinant mouse IL-6 (Cat. No. 575709) as the standard. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.Flow Cytometry:The fluorochrome-labeled MP5-20F3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-6 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.Neutralization1-2,8-10:The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-6 bioactivityin vivoandin vitro(Cat. No. 504506).Additional reported applications (for relevant formats of this clone) include:In VivoCapture7, and immunohistochemical staining (paraformaldehyde-fixed/saponin-treated6, and acetone-fixed frozen tissue sections13, 14).Note:For testing mouse IL-6 in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 431301 to 431306) are specially developed and recommended.
Host
Rat
Reactivity
Mouse
Applications
ELISA Capture -Quality testedCyTOF® -VerifiedELISPOT Capture, Neut, IHC-F -Reported in the literature, not verified in house
Conjugation
Purified anti-mouse IL-6 Antibody anti-IL-6 - MP5-20F3, BioLegend, 504502
ELISA Capture1-3,11or ELISPOT Capture4,5:The purified MP5-20F3 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP5-32C11 antibody (Cat. No. 504602) as the detecting antibody and recombinant mouse IL-6 (Cat. No. 575709) as the standard. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.Flow Cytometry:The fluorochrome-labeled MP5-20F3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-6 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.Neutralization1-2,8-10:The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-6 bioactivityin vivoandin vitro(Cat. No. 504506).Additional reported applications (for relevant formats of this clone) include:In VivoCapture7, and immunohistochemical staining (paraformaldehyde-fixed/saponin-treated6, and acetone-fixed frozen tissue sections13, 14).Note:For testing mouse IL-6 in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 431301 to 431306) are specially developed and recommended.
Host
Rat
Reactivity
Mouse
Applications
ELISA Capture -Quality testedCyTOF® -VerifiedELISPOT Capture, Neut, IHC-F -Reported in the literature, not verified in house
Conjugation
PE anti-mouse IL-6 Antibody anti-IL-6 - MP5-20F3, BioLegend, 504503
ELISA Capture1-3,11or ELISPOT Capture4,5:The purified MP5-20F3 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP5-32C11 antibody (Cat. No. 504602) as the detecting antibody and recombinant mouse IL-6 (Cat. No. 575709) as the standard. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.Flow Cytometry:The fluorochrome-labeled MP5-20F3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-6 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.Neutralization1-2,8-10:The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-6 bioactivityin vivoandin vitro(Cat. No. 504506).Additional reported applications (for relevant formats of this clone) include:In VivoCapture7, and immunohistochemical staining (paraformaldehyde-fixed/saponin-treated6, and acetone-fixed frozen tissue sections13, 14).Note:For testing mouse IL-6 in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 431301 to 431306) are specially developed and recommended.
Host
Rat
Reactivity
Mouse
Applications
ICFC - Quality tested
Conjugation
PE anti-mouse IL-6 Antibody anti-IL-6 - MP5-20F3, BioLegend, 504504
ELISA Capture1-3,11or ELISPOT Capture4,5:The purified MP5-20F3 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP5-32C11 antibody (Cat. No. 504602) as the detecting antibody and recombinant mouse IL-6 (Cat. No. 575709) as the standard. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.Flow Cytometry:The fluorochrome-labeled MP5-20F3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-6 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.Neutralization1-2,8-10:The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-6 bioactivityin vivoandin vitro(Cat. No. 504506).Additional reported applications (for relevant formats of this clone) include:In VivoCapture7, and immunohistochemical staining (paraformaldehyde-fixed/saponin-treated6, and acetone-fixed frozen tissue sections13, 14).Note:For testing mouse IL-6 in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 431301 to 431306) are specially developed and recommended.
Host
Rat
Reactivity
Mouse
Applications
ICFC - Quality tested
Conjugation
APC anti-mouse IL-6 Antibody anti-IL-6 - MP5-20F3, BioLegend, 504507
ELISA Capture1-3,11or ELISPOT Capture4,5:The purified MP5-20F3 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP5-32C11 antibody (Cat. No. 504602) as the detecting antibody and recombinant mouse IL-6 (Cat. No. 575709) as the standard. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.Flow Cytometry:The fluorochrome-labeled MP5-20F3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-6 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.Neutralization1-2,8-10:The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-6 bioactivityin vivoandin vitro(Cat. No. 504506).Additional reported applications (for relevant formats of this clone) include:In VivoCapture7, and immunohistochemical staining (paraformaldehyde-fixed/saponin-treated6, and acetone-fixed frozen tissue sections13, 14).Note:For testing mouse IL-6 in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 431301 to 431306) are specially developed and recommended.
Host
Rat
Reactivity
Mouse
Applications
ICFC - Quality tested
Conjugation
APC anti-mouse IL-6 Antibody anti-IL-6 - MP5-20F3, BioLegend, 504508
ELISA Capture1-3,11or ELISPOT Capture4,5:The purified MP5-20F3 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP5-32C11 antibody (Cat. No. 504602) as the detecting antibody and recombinant mouse IL-6 (Cat. No. 575709) as the standard. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.Flow Cytometry:The fluorochrome-labeled MP5-20F3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-6 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.Neutralization1-2,8-10:The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-6 bioactivityin vivoandin vitro(Cat. No. 504506).Additional reported applications (for relevant formats of this clone) include:In VivoCapture7, and immunohistochemical staining (paraformaldehyde-fixed/saponin-treated6, and acetone-fixed frozen tissue sections13, 14).Note:For testing mouse IL-6 in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 431301 to 431306) are specially developed and recommended.
Host
Rat
Reactivity
Mouse
Applications
ICFC - Quality tested
Conjugation
Biotin anti-mouse IL-6 Antibody anti-IL-6 - MP5-32C11, BioLegend, 504601
ELISA Detection1-4,8or ELISPOT Detection5,6:The biotinylated MP5-32C11 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified MP5-20F3 antibody (Cat. No. 504502/504506) as capture antibody and recombinant mouse IL-6 (Cat. No. 575709) as the standard.Additional reported applications (for the relevant formats) include:Western blotting, In Vivo Capture7.Note:For testing mouse IL-6 in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 431301 to 431306) are specially developed and recommended.
Host
Rat
Reactivity
Mouse
Applications
ELISA Detection, ELISPOT Detection
Conjugation
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