Search results for Ki-67
Brilliant Violet 711 anti-human Ki-67 Antibody anti-Ki-67 - Ki-67, BioLegend, 350515
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1, Western blotting3, and immunofluorescence microscopy4.Ki-67 Staining Protocol:1. Prepare 70% ethanol and chill at -20°C.2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xgfor 5 minutes.3. Discard supernatant and loosen the cell pellet by vortexing.4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 106/ml.7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.
Host
Mouse
Reactivity
Human
Applications
ICFC - Quality tested
Conjugation
Brilliant Violet 711 anti-human Ki-67 Antibody anti-Ki-67 - Ki-67, BioLegend, 350516
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1, Western blotting3, and immunofluorescence microscopy4.Ki-67 Staining Protocol:1. Prepare 70% ethanol and chill at -20°C.2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xgfor 5 minutes.3. Discard supernatant and loosen the cell pellet by vortexing.4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 106/ml.7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.
Host
Mouse
Reactivity
Human
Applications
ICFC - Quality tested
Conjugation
Brilliant Violet 750™ anti-human Ki-67 Antibody, Ki-67, Ki-67, BioLegend, 350535
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1, Western blotting3, and immunofluorescence microscopy4.Ki-67 Staining Protocol:1. Prepare 70% ethanol and chill at -20°C.2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xgfor 5 minutes.3. Discard supernatant and loosen the cell pellet by vortexing.4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 106/ml.7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.
Host
Mouse
Reactivity
Human
Applications
ICFC - Quality tested
Conjugation
Brilliant Violet 750™ anti-human Ki-67 Antibody, Ki-67, Ki-67, BioLegend, 350536
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1, Western blotting3, and immunofluorescence microscopy4.Ki-67 Staining Protocol:1. Prepare 70% ethanol and chill at -20°C.2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xgfor 5 minutes.3. Discard supernatant and loosen the cell pellet by vortexing.4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 106/ml.7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.
Host
Mouse
Reactivity
Human
Applications
ICFC - Quality tested
Conjugation
.png "Click to View PathScan<sup>®</sup>Total Ki-67 Sandwich ELISA Kit#14507 Product Detail")
PathScan®Total Ki-67 Sandwich ELISA Kit#14507, Cell Signaling Technology (CST), 14507
PathScan®Total Ki-67 Sandwich ELISA Kit detects endogenous levels of Ki-67 protein as shown in Figure 1. This kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Host
Reactivity
Human, Monkey
Applications
Conjugation
Ki-67 (D3B5) Rabbit Monoclonal Antibody (Pacific BlueTMConjugate)#52001, Cell Signaling Technology (CST), 52001
Ki-67 (D3B5) Rabbit Monoclonal Antibody (Pacific BlueTM Conjugate) recognizes endogenous levels of total Ki-67 protein.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry (Fixed/Permeabilized): 1:50
Conjugation
Ki-67 (8D5) Mouse Monoclonal Antibody (Alexa Fluor®647 Conjugate)#81240, Cell Signaling Technology (CST), 81240
Ki-67 (8D5) Mouse Monoclonal Antibody (Alexa Fluor®647 Conjugate) recognizes endogenous levels of total Ki-67 protein.
Host
Mouse
Reactivity
Human, Monkey
Applications
Immunohistochemistry (Paraffin): 1:50 - 1:200
Conjugation
Ki-67 (D2H10) Rabbit Monoclonal Antibody (Alexa Fluor®488 Conjugate)#63273, Cell Signaling Technology (CST), 63273
Ki-67 (D2H10) Rabbit Monoclonal Antibody (Alexa Fluor®488 Conjugate) recognizes endogenous levels of total Ki-67 protein.
Host
Rabbit
Reactivity
Human, Monkey
Applications
Immunohistochemistry (Paraffin): 1:100 - 1:400
Conjugation
Ki-67 (D2H10) Rabbit Monoclonal Antibody (Alexa Fluor®555 Conjugate)#88338, Cell Signaling Technology (CST), 88338
Ki-67 (D2H10) Rabbit Monoclonal Antibody (Alexa Fluor®555 Conjugate) recognizes endogenous levels of total Ki-67 protein.
Host
Rabbit
Reactivity
Human, Monkey
Applications
Immunohistochemistry (Paraffin): 1:100 - 1:400
Conjugation
Ki-67 (D2H10) Rabbit Monoclonal Antibody (Alexa Fluor®647 Conjugate)#96344, Cell Signaling Technology (CST), 96344
Ki-67 (D2H10) Rabbit Monoclonal Antibody (Alexa Fluor®647 Conjugate) recognizes endogenous levels of total Ki-67 protein.
Host
Rabbit
Reactivity
Human, Monkey
Applications
Immunohistochemistry (Paraffin): 1:50 - 1:200
Conjugation
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