Search results for Lck antibody
Proto-Oncogene Tyrosine-Protein Kinase LCK (LCK) Antibody, Abbexa, abx034905
Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR) linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP. This antibody is supplied as crude ascites.
Host
Mouse
Reactivity
Human
Applications
ELISA, WB
Conjugation
Unconjugated
Proto-Oncogene Tyrosine-Protein Kinase LCK (LCK) Antibody, Abbexa, abx109389
Tyrosine-protein kinase Lck Antibody is a Rabbit Polyclonal antibody against Tyrosine-protein kinase Lck.
Host
Rabbit
Reactivity
Human
Applications
ELISA, IHC, IF/ICC, IP
Conjugation
Unconjugated
Purified anti-Lck Antibody anti-Lck - LCK-01, BioLegend, 628301
Does not cross-react with mouse.Clone may cross-react with other Src tyrosine kinase family members.
Host
Mouse
Reactivity
Human
Applications
WB -Quality testedICC, IP -VerifiedFC -Reported in the literature, not verified in house
Conjugation
Purified anti-Lck Antibody anti-Lck - LCK-01, BioLegend, 628302
Does not cross-react with mouse.Clone may cross-react with other Src tyrosine kinase family members.
Host
Mouse
Reactivity
Human
Applications
WB -Quality testedICC, IP -VerifiedFC -Reported in the literature, not verified in house
Conjugation
Proto-Oncogene Tyrosine-Protein Kinase LCK (LCK) Antibody, Abbexa, abx327749
LCK Antibody is a Rabbit Polyclonal against LCK.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Applications
ELISA, WB
Conjugation
Unconjugated
![Alexa Fluor® 647 Anti-Lck antibody [Y123] Brand Logo](https://file.buyantibody.com/public/brand/20260608/Abcam.jpg "Click to View Alexa Fluor® 647 Anti-Lck antibody [Y123] Product Detail")
Alexa Fluor® 647 Anti-Lck antibody [Y123], Abcam, AB320622
Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . How are conjugated primary antibodies validated? This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling. For suitable applications and species reactivity, please refer to the unconjugated version of this clone. Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
Host
Rabbit
Reactivity
Human
Applications
Flow Cyt (Intra), Target Binding Affinity, IHC-P, ICC/IF, Antibody Labelling
Conjugation
Alexa Fluor® 647
Proto-oncogene tyrosine-protein kinase LCK (LCK) Antibody (Biotin), Abbexa, abx106038
Tyrosine-protein kinase Lck Antibody (Biotin) is a Rabbit Polyclonal antibody against Tyrosine-protein kinase Lck conjugated to Biotin.
Host
Rabbit
Reactivity
Human
Applications
ELISA
Conjugation
Biotin
Proto-oncogene tyrosine-protein kinase LCK (LCK) Antibody (HRP), Abbexa, abx108872
Tyrosine-protein kinase Lck Antibody (HRP) is a Rabbit Polyclonal antibody against Tyrosine-protein kinase Lck conjugated to HRP.
Host
Rabbit
Reactivity
Human
Applications
ELISA
Conjugation
HRP
Proto-oncogene tyrosine-protein kinase LCK (LCK) Antibody (FITC), Abbexa, abx107452
Tyrosine-protein kinase Lck Antibody (FITC) is a Rabbit Polyclonal antibody against Tyrosine-protein kinase Lck conjugated to FITC.
Host
Rabbit
Reactivity
Human
Applications
Conjugation
FITC
Anti-Lck antibody [EPR27974-49] - BSA and Azide free (Detector), Abcam, AB324343
ab324343 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Lck (phospho Y394). The recommended pair for sandwich ELISA is: Capture: ab324342 , Human Lck (phospho Y394) Capture Antibody (unconjugated) Detector: ab324343, Human Lck (phospho Y394) Detector Antibody (unconjugated) Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Sandwich ELISA The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Host
Rabbit
Reactivity
Human
Applications
sELISA
Conjugation
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