Search results for 387

Spark UV™ 387 anti-mouse CD11c Antibody, CD11c, N418, BioLegend, 117370

Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, immunofluorescence microscopy5, 9(Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10), and spatial biology (IBEX)22,23.

Spark UV™ 387 anti-human CD8 Antibody, CD8, SK1, BioLegend, 344775

Clone SK1 recognizes the a chain of CD8. Additional reported applications (for the relevant formats) include: proteogenomics8, immunohistochemistry of acetone-fixed frozen tissue sections, and spatial biology (IBEX)9,10. This clone was tested in-house and does not demonstrate utility for formalin-fixed paraffin-embedded (FFPE) human tonsil sections.

Spark UV™ 387 anti-human CD8 Antibody, CD8, SK1, BioLegend, 344776

Clone SK1 recognizes the a chain of CD8. Additional reported applications (for the relevant formats) include: proteogenomics8, immunohistochemistry of acetone-fixed frozen tissue sections, and spatial biology (IBEX)9,10. This clone was tested in-house and does not demonstrate utility for formalin-fixed paraffin-embedded (FFPE) human tonsil sections.

Spark UV™ 387 anti-human CD4 Antibody, CD4, SK3, BioLegend, 344685

Spark UV™ 387 anti-human CD4 Antibody, CD4, SK3, BioLegend, 344686

Spark UV™ 387 anti-human CD45RO Antibody, CD45RO, UCHL1, BioLegend, 304271

The UCHL1 antibody is commonly used in combination with antibodies against CD45RA to discern memory and naïve T cells. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5and formalin-fixed paraffin-embedded tissue sections4, Western blotting2, and immunoprecipitation3.UCHL1 signal intensity may be reduced in the presence of True-Stain Monocyte Blocker™ (Cat. No. 426102). Clone S19021B is not impacted by Monocyte Blocker and is recommended as an alternative to UCHL1.

Spark UV™ 387 anti-human CD45RO Antibody, CD45RO, UCHL1, BioLegend, 304272

The UCHL1 antibody is commonly used in combination with antibodies against CD45RA to discern memory and naïve T cells. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5and formalin-fixed paraffin-embedded tissue sections4, Western blotting2, and immunoprecipitation3.UCHL1 signal intensity may be reduced in the presence of True-Stain Monocyte Blocker™ (Cat. No. 426102). Clone S19021B is not impacted by Monocyte Blocker and is recommended as an alternative to UCHL1.

Spark UV™ 387 anti-mouse CD90.2 (Thy1.2) Antibody, CD90.2, S20008D, BioLegend, 164803

Clone S20008D antibody reacts with mouse CD90.2 (Thy 1.2) which is expressed by C57BL/6 and BALB/C strains, but not on CD90.1 strains (AKR/J, PL, FVB, etc.).Clone S20008D can completely block binding of clone 30-H12 on target cells.To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with TruStain FcX™ PLUS (anti-mouse CD16/32) Antibody (Cat. No. 156603 & 156604) is recommended prior to staining.

Spark UV™ 387 anti-mouse CD90.2 (Thy1.2) Antibody, CD90.2, S20008D, BioLegend, 164804

Clone S20008D antibody reacts with mouse CD90.2 (Thy 1.2) which is expressed by C57BL/6 and BALB/C strains, but not on CD90.1 strains (AKR/J, PL, FVB, etc.).Clone S20008D can completely block binding of clone 30-H12 on target cells.To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with TruStain FcX™ PLUS (anti-mouse CD16/32) Antibody (Cat. No. 156603 & 156604) is recommended prior to staining.

Spark UV™ 387 anti-human CD235a (Glycophorin A) Antibody, CD235a, HI264, BioLegend, 349133