Search results for 550
Spark Blue™ 550 anti-human IgM Antibody, IgM, MHM-88, BioLegend, 314555
MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Spark Blue™ 550 anti-human IgM Antibody, IgM, MHM-88, BioLegend, 314556
MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Spark Blue™ 550 anti-mouse CD45 Antibody, CD45, 30-F11, BioLegend, 103165
Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).
Host
Rat
Reactivity
Mouse
Applications
FC -Quality tested
Conjugation
Spark Blue™ 550 anti-mouse CD45 Antibody, CD45, 30-F11, BioLegend, 103166
Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).
Host
Rat
Reactivity
Mouse
Applications
FC -Quality tested
Conjugation
Spark Blue™ 550 anti-human CD36 Antibody, CD36, 5-271, BioLegend, 336241
Additional reported applications (for the relevant formats) include: immunofluorescence2.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Spark Blue™ 550 anti-human CD36 Antibody, CD36, 5-271, BioLegend, 336242
Additional reported applications (for the relevant formats) include: immunofluorescence2.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Spark Blue™ 550 anti-mouse CD8a Antibody, CD8a, 53-6.7, BioLegend, 100779
Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3,in vivoandin vitrocell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, immunohistochemical staining5,6of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).
Host
Rat
Reactivity
Mouse
Applications
FC -Quality tested
Conjugation
Spark Blue™ 550 anti-mouse CD8a Antibody, CD8a, 53-6.7, BioLegend, 100780
Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3,in vivoandin vitrocell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, immunohistochemical staining5,6of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).
Host
Rat
Reactivity
Mouse
Applications
FC -Quality tested
Conjugation
Spark Blue™ 550 anti-human CD244 (2B4) Antibody, CD244, C1.7, BioLegend, 329545
Additional reported applications (for the relevant formats) include: inducing cytokine production by NK cells, enhancing NK cytotoxicity, and Western blotting.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Spark Blue™ 550 anti-human CD244 (2B4) Antibody, CD244, C1.7, BioLegend, 329546
Additional reported applications (for the relevant formats) include: inducing cytokine production by NK cells, enhancing NK cytotoxicity, and Western blotting.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
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