Search results for H2A.X

FITC anti-H2A.X Phospho Ser139 Antibody anti-Phosphorylated H2A.X - 2F3, BioLegend, 613403

Additional reported applications (for the relevant formats of this clone) include:immunohistochemistry on paraffin embedded sections2, immunofluorescence microscopy3-9, Western blotting10-12, and flow cytometry1,13. Clone 2F3 cross-reacts with mouse4.Intracellular staining protocol for Anti-H2A.X-Phosphorylated (Ser139) Antibodyfor Flow Cytometry1. Prepare 70% absolute ethanol. Chill solution by storing at -20°C.2. Prepare cells of interest.3. Wash 1X with PBS, centrifuge at 350g for 5 min.4. Discard the supernatant and vortex to loosen cell pellet.5. Add pre-cooled 70% ethanol drop by drop, while vortexing.6. Incubate at -20°C for 60 minutes.7. Wash 3X withBioLegend Cell Staining Bufferand resuspend the cells at 0.5-1 X 107cells/ml in the cell staining buffer.8. Perform immunofluorescent staining for flow cytometry.

FITC anti-H2A.X Phospho Ser139 Antibody anti-Phosphorylated H2A.X - 2F3, BioLegend, 613404

Additional reported applications (for the relevant formats of this clone) include:immunohistochemistry on paraffin embedded sections2, immunofluorescence microscopy3-9, Western blotting10-12, and flow cytometry1,13. Clone 2F3 cross-reacts with mouse4.Intracellular staining protocol for Anti-H2A.X-Phosphorylated (Ser139) Antibodyfor Flow Cytometry1. Prepare 70% absolute ethanol. Chill solution by storing at -20°C.2. Prepare cells of interest.3. Wash 1X with PBS, centrifuge at 350g for 5 min.4. Discard the supernatant and vortex to loosen cell pellet.5. Add pre-cooled 70% ethanol drop by drop, while vortexing.6. Incubate at -20°C for 60 minutes.7. Wash 3X withBioLegend Cell Staining Bufferand resuspend the cells at 0.5-1 X 107cells/ml in the cell staining buffer.8. Perform immunofluorescent staining for flow cytometry.

Anti-Histone H2A.X antibody, Abcam, AB11175

What is this antibody validated in? Anti-Histone H2A.X antibody (ab11175) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse samples. Trusted by the scientific community Anti-Histone H2A.X (ab11175) was first used in a scientific publication in 2004 and has been cited over 150 times in peer-reviewed journals. Reviewed by scientists Anti-Histone H2A.X (ab11175) has over 40 independent reviews from customers.

Anti-Histone H2A.X antibody, Abcam, AB140498

Anti-Histone H2A.X antibody, Abcam, AB209980

Non-phospho Ser139

Histone H2A.X (H2AX) Antibody, Abbexa, abx233885

Histone H2A.X (H2AX) Antibody is a Rabbit Polyclonal against Histone H2A.X (H2AX).

Histone H2A.X (pS139) Antibody, Abbexa, abx119287

Histone H2A.X (pS139) Antibody is a Rabbit Polyclonal antibody against Histone H2A.X (pS139).

Histone H2A.X (H2AX) Antibody, Abbexa, abx109878

Histone H2A.

Histone H2A.X (H2AFX) Antibody, Abbexa, abx034840

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Histone H2A.X Antibody (YA380), MedChemexpress, HY-P80161

Histone H2A.X Antibody (YA380) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Histone H2A.X.