Search results for 538
Spark Violet™ 538 anti-mouse I-A/I-E, I-A/I-E Antibody, M5/114.15.2, BioLegend, 107671
The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-EkMHC class II alloantigens from mice carrying H-2p,r,q,b,d,uhaplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-AsMHC class II alloantigens.1Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6,in vitroandin vivoblocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
Spark Violet™ 538 anti-mouse I-A/I-E, I-A/I-E Antibody, M5/114.15.2, BioLegend, 107672
The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-EkMHC class II alloantigens from mice carrying H-2p,r,q,b,d,uhaplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-AsMHC class II alloantigens.1Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6,in vitroandin vivoblocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
Spark Violet™ 538 anti-human Ig light chain κ Antibody, Ig light chain κ, TB28-2, BioLegend, 392713
The TB28-2 target antigen is also present in serum. Cell surface staining may be optimized by removing serum from the sample prior to staining.Additional reported applications (for relevant formats) include immunohistochemical staining on frozen tissue sections1.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Spark Violet™ 538 anti-human Ig light chain κ Antibody, Ig light chain κ, TB28-2, BioLegend, 392714
The TB28-2 target antigen is also present in serum. Cell surface staining may be optimized by removing serum from the sample prior to staining.Additional reported applications (for relevant formats) include immunohistochemical staining on frozen tissue sections1.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Spark Violet™ 538 anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody, Ly-6G/Ly-6C, RB6-8C5, BioLegend, 108475
Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C19. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A819. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.420.The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytesin vivo. Additional reported applications (for relevant formats of this clone) include:in vitrocomplement-mediated cytotoxicity2,in vivodepletion3-5,9, immunoprecipitation1, immunohistochemical staining6(including paraffin-embedded sections9,16,33-35, acetone-fixed frozen sections11and zinc-fixed sections15), and Western blotting7. RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs)20.Special Note:Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 108436).
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
Spark Violet™ 538 anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody, Ly-6G/Ly-6C, RB6-8C5, BioLegend, 108476
Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C19. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A819. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.420.The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytesin vivo. Additional reported applications (for relevant formats of this clone) include:in vitrocomplement-mediated cytotoxicity2,in vivodepletion3-5,9, immunoprecipitation1, immunohistochemical staining6(including paraffin-embedded sections9,16,33-35, acetone-fixed frozen sections11and zinc-fixed sections15), and Western blotting7. RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs)20.Special Note:Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 108436).
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
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