Search results for BrdU
BrdU(Proliferation Marker) Antibody (YA6399)(PBS only), MedChemexpress, HY-P86707A
BrdU(Proliferation Marker) Antibody (YA6399) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to BrdU(Proliferation Marker).
Host
Mouse
Reactivity
Species independent
Applications
ICC/IF
Conjugation
Non-conjugated
.png "Click to View BrdU (Bu20a) Mouse Monoclonal Antibody (PE Conjugate)#50230 Product Detail")
BrdU (Bu20a) Mouse Monoclonal Antibody (PE Conjugate)#50230, Cell Signaling Technology (CST), 50230
BrdU (Bu20a) Mouse Monoclonal Antibody (PE Conjugate) detects BrdU when incorporated into single stranded DNA. DNA must be denatured for the epitope to be exposed and recognized by the antibody.
Host
Mouse
Reactivity
All Species Expected
Applications
Flow Cytometry (Fixed/Permeabilized): 1:50
Conjugation
![Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker Brand Logo](https://file.buyantibody.com/public/brand/20260608/Abcam.jpg "Click to View Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker Product Detail")
Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker, Abcam, AB6326
Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326) is a rat monoclonal antibody and is validated for use in Flow Cyt (Intra), ICC/IF and IHC-P. Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326) was first used in a scientific publication in 1985 and has been cited over 1925 times in peer reviewed journals. It's performance in IHC in mouse samples is trusted by the scientific community. Abcam's high quality validation processes ensure Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326) has high sensitivity and specificity. Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326) has 105 independent reviews from customers. Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326) specifically detects BrdU (UniProt ID: N/A; Molecular weight: 0.3kDa) and is sold in 100 ug and 1 mg selling sizes. Conjugation-ready, carrier free format available for antibody clone BU1/75 (ICR1) - ab264079 . Antibody clone BU1/75 (ICR1) is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor ® 488, Alexa Fluor ® 647, Alexa Fluor ® 594, HRP, Alexa Fluor ® 555 ( ab2274 , ab2275 , ab2276 , ab2257 , ab22124 ). Top cited antibody on the market for the proliferation marker BrdU with >2400 citations. ab6326 has over 80 5 star reviews. BrdU antibody is essential for accurately tracking cell proliferation and DNA synthesis in various research applications, including cancer and neurogenesis studies
Host
Rat
Reactivity
Applications
ICC/IF, IHC-P, Flow Cyt (Intra)
Conjugation
Anti-BrdU antibody [BU1/75 (ICR1)] - BSA and Azide free, Abcam, AB264079
ab264079 is the carrier-free version of ab6326 . Protocol advice: This antibody recognizes single stranded DNA so the DNA needs to be unraveled first. This can be done with DNAse, although it doesn't give the best results. Depending on the assay, acid denaturation with 2M HCL or heat denaturation are the most successful. Please note this step is critical in any assay with this antibody and is the area that should be modified to optimize results. A detailed BrdU staining protocol is available in the Protocols tab or by clicking on this link . Unstained positive control slides from mice treated with BrdU (formalin-fixed, paraffin-embedded intestine sections) are available as BrdU control slides ab129956 . AF488 conjugate available as ab220074 AF647 conjugate available as ab220075 Please see the Associated Products tab for other available conjugates. Want a custom formulation? This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact [email protected] Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Compatibility This product is compatible with the Maxpar ® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar ® is a trademark of Fluidigm Canada Inc.
Host
Rat
Reactivity
Applications
Flow Cyt (Intra), ICC/IF, IHC-P
Conjugation
Alexa Fluor® 488 Anti-BrdU antibody [BU1/75 (ICR1)], Abcam, AB220074
What is this antibody validated in? Alexa Fluor® 488 Anti-BrdU antibody [BU1/75 (ICR1)] (ab220074) is a rat monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF). Related products Antibody clone BU1/75 (ICR1) is also available pre-conjugated to a variety of labels for your convenience – Anti-BrdU Alexa Fluor® 488 [BU1/75 (ICR1)] (ab220074). Other related products We have a range of other formats of antibody clone [BU1/75 (ICR1)] also available for your convenience: ab6326 , Alexa Fluor® 488 - ab220074, Alexa Fluor® 647 - ab220075 , Alexa Fluor® 594 - ab220076 , HRP - ab220507 , Alexa Fluor® 555 - ab221240 , Carrier free - ab264079 Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
Host
Rat
Reactivity
Applications
ICC/IF
Conjugation
Alexa Fluor® 488
Alexa Fluor® 555 Anti-BrdU antibody [BU1/75 (ICR1)], Abcam, AB221240
The antibody recognises single stranded DNA so the DNA needs to be unraveled first. This can be done with DNAse, although this doesn't give the best results. Depending on the assay, acid denaturation with 2M HCL or heat denaturation are the most successful. Please note this step is critical in any assay with this antibody and is the area that should be modified to optimise results. Detailed BrdU protocol is available here . Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
Host
Rat
Reactivity
Applications
ICC/IF
Conjugation
Alexa Fluor® 555
Alexa Fluor® 594 Anti-BrdU antibody [BU1/75 (ICR1)], Abcam, AB220076
The antibody recognises single stranded DNA so the DNA needs to be unraveled first. This can be done with DNAse, although this doesn't give the best results. Depending on the assay, acid denaturation with 2M HCL or heat denaturation are the most successful. Please note this step is critical in any assay with this antibody and is the area that should be modified to optimise results. Detailed BrdU protocol is available here . Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
Host
Rat
Reactivity
Applications
ICC/IF
Conjugation
Alexa Fluor® 594
Alexa Fluor® 647 Anti-BrdU antibody [BU1/75 (ICR1)], Abcam, AB220075
The antibody recognises single stranded DNA so the DNA needs to be unraveled first. This can be done with DNAse, although this doesn't give the best results. Depending on the assay, acid denaturation with 2M HCL or heat denaturation are the most successful. Please note this step is critical in any assay with this antibody and is the area that should be modified to optimise results. Detailed BrdU protocol is available here . Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
Host
Rat
Reactivity
Applications
ICC/IF
Conjugation
Alexa Fluor® 647
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