Search results for NKG2D antibody
Purified anti-human CD314 NKG2D Antibody anti-CD314 - 1D11, BioLegend, 320802
The 1D11 antibody blocks MICA binding to T cells, induces redirected lysis, and costimulates T cells activation and proliferation. Additional reported (for the relevant formats) applications include: immunoprecipitation1,2, blocking of ligand binding, induction of redirected cell lysis, and costimulation of T cells proliferation2-7. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 320814) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.
Host
Mouse
Reactivity
Human
Applications
FC - Quality testedIP - Reported in the literature, not verified in house
Conjugation
TotalSeq-B0165 anti-human CD314 NKG2D Antibody, CD314, 1D11, BioLegend, 320839
The 1D11 antibody blocks MICA binding to T cells, induces redirected lysis, and costimulates T cells activation and proliferation. Additional reported (for the relevant formats) applications include: immunoprecipitation1,2, blocking of ligand binding, induction of redirected cell lysis, and costimulation of T cells proliferation2-7. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 320814) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.
Host
Mouse
Reactivity
Human
Applications
PG -Quality tested
Conjugation
PE anti-mouse CD314 NKG2D Antibody anti-CD314 - A10, BioLegend, 115605
Additional reported applications include: blocking and inducing redirected cell lysis1,2. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 115606) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 115610).
Host
Armenian Hamster
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
PE anti-mouse CD314 NKG2D Antibody anti-CD314 - A10, BioLegend, 115606
Additional reported applications include: blocking and inducing redirected cell lysis1,2. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 115606) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 115610).
Host
Armenian Hamster
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
Biotin anti-mouse CD314 NKG2D Antibody anti-CD314 - C7, BioLegend, 115703
Additional reported (for the relevant formats) applications include: blocking1,2,4,5and inducing redirected cell lysis1,2. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 115706) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 115704) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)). The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 115713 & 115714).
Host
Armenian Hamster
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
FITC anti-mouse CD314 NKG2D Antibody anti-CD314 - C7, BioLegend, 115711
Additional reported (for the relevant formats) applications include: blocking1,2,4,5and inducing redirected cell lysis1,2. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 115706) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 115704) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)). The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 115713 & 115714).
Host
Armenian Hamster
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
PE anti-mouse CD314 NKG2D Antibody anti-CD314 - C7, BioLegend, 115705
Additional reported (for the relevant formats) applications include: blocking1,2,4,5and inducing redirected cell lysis1,2. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 115706) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 115704) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)). The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 115713 & 115714).
Host
Armenian Hamster
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
PE anti-mouse CD314 NKG2D Antibody anti-NKG2D - CX5, BioLegend, 130207
To reduce non-specific binding, preincubate cells with purified anti-mouse CD16/CD32, clone 93 (Cat No 101301/2) is recommended.This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes contact The Regents of the University of California.
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
PE anti-mouse CD314 NKG2D Antibody anti-NKG2D - CX5, BioLegend, 130208
To reduce non-specific binding, preincubate cells with purified anti-mouse CD16/CD32, clone 93 (Cat No 101301/2) is recommended.This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes contact The Regents of the University of California.
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
APC anti-mouse CD314 NKG2D Antibody anti-NKG2D - CX5, BioLegend, 130211
To reduce non-specific binding, preincubate cells with purified anti-mouse CD16/CD32, clone 93 (Cat No 101301/2) is recommended.This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes contact The Regents of the University of California.
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
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