Search results for APC/Fire
APC/Fire™ 750 anti-human CD3 Antibody CD3, OKT3, BioLegend, 317352
The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex.Clone OKT3 can block the binding of clones SK7 and UCHT1.4The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Host
Mouse
Reactivity
Human
Applications
FC -Quality tested
Conjugation
APC/Fire™ 750 anti-human CD4 Antibody, CD4, OKT4, BioLegend, 317461
The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317453 and 317454).In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.9
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Applications
FC - Quality tested
Conjugation
APC/Fire™ 750 anti-human CD4 Antibody, CD4, OKT4, BioLegend, 317462
The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317453 and 317454).In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.9
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Applications
FC - Quality tested
Conjugation
APC/Fire 750 anti-mouse CD3 Antibody anti-CD3 - 17A2, BioLegend, 100247
Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
APC/Fire 750 anti-mouse CD3 Antibody anti-CD3 - 17A2, BioLegend, 100248
Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
APC/Fire™ 810 anti-mouse CD3 Antibody, CD3, 17A2, BioLegend, 100267
Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.
Host
Rat
Reactivity
Mouse
Applications
FC -Quality tested
Conjugation
APC/Fire™ 810 anti-mouse CD3 Antibody, CD3, 17A2, BioLegend, 100268
Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.
Host
Rat
Reactivity
Mouse
Applications
FC -Quality tested
Conjugation
APC/Fire 750 anti-human ROR1 Antibody anti-ROR1 - 2A2, BioLegend, 357813
Clone 2A2 binds to the membrane distal Ig-domain of ROR1. Clone 2A2 has also been shown to exhibit cross reactivity towards mouse ROR12.
Host
Mouse
Reactivity
Human
Applications
FC -Quality tested
Conjugation
APC/Fire 750 anti-human ROR1 Antibody anti-ROR1 - 2A2, BioLegend, 357814
Clone 2A2 binds to the membrane distal Ig-domain of ROR1. Clone 2A2 has also been shown to exhibit cross reactivity towards mouse ROR12.
Host
Mouse
Reactivity
Human
Applications
FC -Quality tested
Conjugation
APC/Fire 750 anti-human CD45 Antibody anti-CD45 - 2D1, BioLegend, 368517
It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
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