Search results for H2A.X
PerCP/Cyanine5.5 anti-H2A.X-Phosphorylated Ser139 Antibody anti-Phosphorylated H2A.X - 2F3, BioLegend, 613414
Additional reported applications (for the relevant formats of this clone) include:immunohistochemistry on paraffin embedded sections2, immunofluorescence microscopy3-9, Western blotting10-12, and flow cytometry1,13. Clone 2F3 cross-reacts with mouse4.Intracellular staining protocol for Anti-H2A.X-Phosphorylated (Ser139) Antibodyfor Flow Cytometry1. Prepare 70% absolute ethanol. Chill solution by storing at -20°C.2. Prepare cells of interest.3. Wash 1X with PBS, centrifuge at 350g for 5 min.4. Discard the supernatant and vortex to loosen cell pellet.5. Add pre-cooled 70% ethanol drop by drop, while vortexing.6. Incubate at -20°C for 60 minutes.7. Wash 3X withBioLegend Cell Staining Bufferand resuspend the cells at 0.5-1 X 107cells/ml in the cell staining buffer.8. Perform immunofluorescent staining for flow cytometry.
Host
Mouse
Reactivity
Human, Mouse
Applications
ICFC - Quality tested
Conjugation
Alexa Fluor 488 anti-H2A.X Phospho Ser139 Antibody anti-Phosphorylated H2A.X - 2F3, BioLegend, 613405
Additional reported applications (for the relevant formats of this clone) include:immunohistochemistry on paraffin embedded sections2, immunofluorescence microscopy3-9, Western blotting10-12, and flow cytometry1,13. Clone 2F3 cross-reacts with mouse4.Intracellular staining protocol for Anti-H2A.X-Phosphorylated (Ser139) Antibodyfor Flow Cytometry1. Prepare 70% absolute ethanol. Chill solution by storing at -20°C.2. Prepare cells of interest.3. Wash 1X with PBS, centrifuge at 350g for 5 min.4. Discard the supernatant and vortex to loosen cell pellet.5. Add pre-cooled 70% ethanol drop by drop, while vortexing.6. Incubate at -20°C for 60 minutes.7. Wash 3X withBioLegend Cell Staining Bufferand resuspend the cells at 0.5-1 X 107cells/ml in the cell staining buffer.8. Perform immunofluorescent staining for flow cytometry.
Host
Mouse
Reactivity
Human, Mouse
Applications
ICFC -Quality tested
Conjugation
Alexa Fluor 488 anti-H2A.X Phospho Ser139 Antibody anti-Phosphorylated H2A.X - 2F3, BioLegend, 613406
Additional reported applications (for the relevant formats of this clone) include:immunohistochemistry on paraffin embedded sections2, immunofluorescence microscopy3-9, Western blotting10-12, and flow cytometry1,13. Clone 2F3 cross-reacts with mouse4.Intracellular staining protocol for Anti-H2A.X-Phosphorylated (Ser139) Antibodyfor Flow Cytometry1. Prepare 70% absolute ethanol. Chill solution by storing at -20°C.2. Prepare cells of interest.3. Wash 1X with PBS, centrifuge at 350g for 5 min.4. Discard the supernatant and vortex to loosen cell pellet.5. Add pre-cooled 70% ethanol drop by drop, while vortexing.6. Incubate at -20°C for 60 minutes.7. Wash 3X withBioLegend Cell Staining Bufferand resuspend the cells at 0.5-1 X 107cells/ml in the cell staining buffer.8. Perform immunofluorescent staining for flow cytometry.
Host
Mouse
Reactivity
Human, Mouse
Applications
ICFC -Quality tested
Conjugation
Alexa Fluor 647 anti-H2A.X Phospho Ser139 Antibody anti-Phosphorylated H2A.X - 2F3, BioLegend, 613407
Additional reported applications (for the relevant formats of this clone) include:immunohistochemistry on paraffin embedded sections2, immunofluorescence microscopy3-9, Western blotting10-12, and flow cytometry1,13. Clone 2F3 cross-reacts with mouse4.Intracellular staining protocol for Anti-H2A.X-Phosphorylated (Ser139) Antibodyfor Flow Cytometry1. Prepare 70% absolute ethanol. Chill solution by storing at -20°C.2. Prepare cells of interest.3. Wash 1X with PBS, centrifuge at 350g for 5 min.4. Discard the supernatant and vortex to loosen cell pellet.5. Add pre-cooled 70% ethanol drop by drop, while vortexing.6. Incubate at -20°C for 60 minutes.7. Wash 3X withBioLegend Cell Staining Bufferand resuspend the cells at 0.5-1 X 107cells/ml in the cell staining buffer.8. Perform immunofluorescent staining for flow cytometry.
Host
Mouse
Reactivity
Human, Mouse
Applications
ICFC -Quality tested
Conjugation
Alexa Fluor 647 anti-H2A.X Phospho Ser139 Antibody anti-Phosphorylated H2A.X - 2F3, BioLegend, 613408
Additional reported applications (for the relevant formats of this clone) include:immunohistochemistry on paraffin embedded sections2, immunofluorescence microscopy3-9, Western blotting10-12, and flow cytometry1,13. Clone 2F3 cross-reacts with mouse4.Intracellular staining protocol for Anti-H2A.X-Phosphorylated (Ser139) Antibodyfor Flow Cytometry1. Prepare 70% absolute ethanol. Chill solution by storing at -20°C.2. Prepare cells of interest.3. Wash 1X with PBS, centrifuge at 350g for 5 min.4. Discard the supernatant and vortex to loosen cell pellet.5. Add pre-cooled 70% ethanol drop by drop, while vortexing.6. Incubate at -20°C for 60 minutes.7. Wash 3X withBioLegend Cell Staining Bufferand resuspend the cells at 0.5-1 X 107cells/ml in the cell staining buffer.8. Perform immunofluorescent staining for flow cytometry.
Host
Mouse
Reactivity
Human, Mouse
Applications
ICFC -Quality tested
Conjugation
Alexa Fluor 594 anti-H2A.X Phospho Ser139 Antibody anti-Phosphorylated H2A.X - 2F3, BioLegend, 613410
Additional reported applications (for the relevant formats of this clone) include:immunohistochemistry on paraffin embedded sections2, immunofluorescence microscopy3-9, Western blotting10-12, and flow cytometry1,13. Clone 2F3 cross-reacts with mouse4.Intracellular staining protocol for Anti-H2A.X-Phosphorylated (Ser139) Antibodyfor Flow Cytometry1. Prepare 70% absolute ethanol. Chill solution by storing at -20°C.2. Prepare cells of interest.3. Wash 1X with PBS, centrifuge at 350g for 5 min.4. Discard the supernatant and vortex to loosen cell pellet.5. Add pre-cooled 70% ethanol drop by drop, while vortexing.6. Incubate at -20°C for 60 minutes.7. Wash 3X withBioLegend Cell Staining Bufferand resuspend the cells at 0.5-1 X 107cells/ml in the cell staining buffer.8. Perform immunofluorescent staining for flow cytometry.
Host
Mouse
Reactivity
Human, Mouse
Applications
ICC -Quality tested
Conjugation
![Anti-Histone H2A.X antibody [RM1271] Brand Logo](https://file.buyantibody.com/public/brand/20260608/Abcam.jpg "Click to View Anti-Histone H2A.X antibody [RM1271] Product Detail")
Anti-Histone H2A.X antibody [RM1271], Abcam, AB322655
What are recombinant multiclonals? Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including: - The sensitivity of polyclonal antibodies by recognising multiple epitopes - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production View our range of recombinant multiclonal antibodies . Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents .
Host
Rabbit
Reactivity
Human, Mouse, Rat
Applications
Flow Cyt (Intra), IP, ICC/IF, PepArr, WB, IHC-P
Conjugation
Histone H2A.X (H2AX) Antibody (Biotin), Abbexa, abx105525
Histone H2A.
Host
Rabbit
Reactivity
Human
Applications
ELISA
Conjugation
Biotin
Histone H2A.X (H2AX) Antibody (HRP), Abbexa, abx108363
Histone H2A.
Host
Rabbit
Reactivity
Human
Applications
ELISA
Conjugation
HRP
Histone H2A.X (H2AX) Antibody (FITC), Abbexa, abx106942
Histone H2A.
Host
Rabbit
Reactivity
Human
Applications
Conjugation
FITC
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