Conjugated Antibodies

Conjugated antibodies are primary antibodies covalently linked to detection labels via chemical conjugation, allowing signal detection without secondary antibodies.Benefits include direct detection, lower background, simplified procedures, and more accurate quantification.
Common labels include:
Enzymatic labels (HRP, AP)
Fluorescent labels (FITC, Cy3, Alexa Fluor, PE, APC)
Biotin
Oligonucleotide barcodes (for high-throughput assays such as Olink)
While multiplex staining flexibility is limited and costs are higher than conventional antibody systems, conjugated antibodies deliver minimal background and fast workflows, making them ideal for high-background samples, rapid diagnostics, in vivo imaging, and multicolor flow cytometry.