Flow Cytometry Antibodies

Flow Cytometry Antibodies are fluorescence-conjugated or metal-tagged antibodies optimized for flow cytometric analysis, enabling rapid multiparametric quantification of target proteins and cell sorting in single-cell suspensions. Key features include high brightness, low background, multicolor compatibility, and quantitative accuracy. Commonly used fluorochromes span classic dyes (FITC, PE, PerCP-Cy5.5, APC), photostable Alexa Fluor series, violet laser-excitable Brilliant Violet dyes, and tandem conjugates (PE-Cy7/APC-Cy7). Primary applications encompass immunophenotyping (e.g., CD3⁺CD4⁺ T cell identification), intracellular cytokine detection (IFN-γ, IL-2), proliferation/apoptosis assays (CFSE, Ki-67, Annexin V), phospho-flow, fluorescence-activated cell sorting (FACS), and microparticle analysis, serving as essential tools for modern high-throughput single-cell analysis.