Primary Antibodies

In immunoassays, the primary antibody serves as the core "probe" for precise molecular recognition. As a specific antibody (typically an immunoglobulin such as IgG or IgM) that binds directly to target antigens (e.g., proteins, peptides), it provides the critical specificity foundation for techniques including Western Blot, immunohistochemistry (IHC), and immunofluorescence (IF) by recognizing distinct epitopes. The quality of the primary antibody directly determines the accuracy and reliability of detection results, making it the pivotal element for successful immunodetection experiments.