Western Blot Antibodies are antibodies for immunoblotting applications, capable of recognizing linear epitopes under denaturing conditions to enable qualitative and semi-quantitative analysis of target proteins. Key experimental steps include blocking (5% non-fat milk or BSA to minimize non-specific binding), antibody dilution (primary antibody typically 1:1000–1:10000 for optimal signal-to-noise ratio), thorough washing (TBST to remove unbound antibodies), matched secondary antibody (HRP- or fluorophore-conjugated, species-specific), and loading control normalization (β-actin, GAPDH, etc.). Primary applications encompass protein expression analysis (validation of overexpression or knockdown efficiency), post-translational modification detection (phosphorylation, acetylation status), protein-protein interaction verification (detection of co-immunoprecipitated proteins), biomarker screening (differential expression of disease-associated proteins), and antibody quality control, serving as fundamental tools in molecular biology and protein research.
SOD3 is a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. This protein is thought to protect the brain, lungs, and other tissues from oxidative stress. The protein is secreted into the extracellular space and forms a glycosylated homotetramer that is anchored to the extracellular matrix (ECM) and cell surfaces through an interaction with heparan sulfate proteoglycan and collagen. A fraction of the protein is cleaved near the C-terminus before secretion to generate circulating tetramers that do not interact with the ECM.
This product is currently in development. The lead time for this product may be several months. Please contact us at [email protected] for an updated lead time before purchasing this product. Human Transforming Growth Factor Alpha (TGFA) Antibody is a Mouse monoclonal against Transforming Growth Factor Alpha (TGFA).
This gene encodes two proteins: sterol carrier protein X (SCPx) and sterol carrier protein 2 (SCP2), as a result of transcription initiation from 2 independently regulated promoters. The transcript initiated from the proximal promoter encodes the longer SCPx protein, and the transcript initiated from the distal promoter encodes the shorter SCP2 protein, with the 2 proteins sharing a common C-terminus. Evidence suggests that the SCPx protein is a peroxisome-associated thiolase that is involved in the oxidation of branched chain fatty acids, while the SCP2 protein is thought to be an intracellular lipid transfer protein. This gene is highly expressed in organs involved in lipid metabolism, and may play a role in Zellweger syndrome, in which cells are deficient in peroxisomes and have impaired bile acid synthesis. Alternative splicing of this gene produces multiple transcript variants, some encoding different isoforms.
Cytoplasmic dynein is a microtubule-associated motor protein (Hughes et al., 1995 [PubMed 7738094]). See DYNC1H1 (MIM 600112) for general information about dyneins.
Host
Rabbit
Reactivity
Human
Applications
ELISA, WB, IHC
Conjugation
Unconjugated
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