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Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb (SignalFlexTM Alexa Fluor®750 Conjugate) detects endogenous levels of p44 and p42 MAP Kinase (Erk1 and Erk2) when dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), but not singly phosphorylated at Thr202 or Tyr204. This antibody does not cross-react with the corresponding phosphorylated residues of either SAPK/JNK or p38 MAP kinase. This antibody may cross-react with an unknown cytoskeletal protein in some cell lines as visualized by immunofluorescence.
Host
Mouse
Reactivity
Human, Mouse, Rat, Hamster, Monkey, Mink, Zebrafish Antibodies, Bovine, Pig
Applications
Conjugation
SignalFlexTMAlexa Fluor®750 Conjugate
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Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb (SignalFlexTM mFluorTM Red 780 Conjugate) detects endogenous levels of p44 and p42 MAP Kinase (Erk1 and Erk2) when dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), but not singly phosphorylated at Thr202 or Tyr204. This antibody does not cross-react with the corresponding phosphorylated residues of either SAPK/JNK or p38 MAP kinase. This antibody may cross-react with an unknown cytoskeletal protein in some cell lines as visualized by immunofluorescence.
Host
Mouse
Reactivity
Human, Mouse, Rat, Hamster, Monkey, Mink, Zebrafish Antibodies, Bovine, Pig
Applications
Conjugation
SignalFlexTMmFluorTMRed 780 Conjugate
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Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb (SignalFlexTM mFluorTM UV375 Conjugate) detects endogenous levels of p44 and p42 MAP Kinase (Erk1 and Erk2) when dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), but not singly phosphorylated at Thr202 or Tyr204. This antibody does not cross-react with the corresponding phosphorylated residues of either SAPK/JNK or p38 MAP kinase. This antibody may cross-react with an unknown cytoskeletal protein in some cell lines as visualized by immunofluorescence.
Host
Mouse
Reactivity
Human, Mouse, Rat, Hamster, Monkey, Mink, Zebrafish Antibodies, Bovine, Pig
Applications
Conjugation
SignalFlexTMmFluorTMUV375 Conjugate
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Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb (SignalFlexTM mFluorTM Violet 510 Conjugate) detects endogenous levels of p44 and p42 MAP Kinase (Erk1 and Erk2) when dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), but not singly phosphorylated at Thr202 or Tyr204. This antibody does not cross-react with the corresponding phosphorylated residues of either SAPK/JNK or p38 MAP kinase. This antibody may cross-react with an unknown cytoskeletal protein in some cell lines as visualized by immunofluorescence.
Host
Mouse
Reactivity
Human, Mouse, Rat, Hamster, Monkey, Mink, Zebrafish Antibodies, Bovine, Pig
Applications
Conjugation
SignalFlexTMmFluorTMViolet 510 Conjugate
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Acetyl-alpha-Tubulin (Lys40) (D20G3) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of α-tubulin only when acetylated at Lys40. This amino acid is not conserved in β-tubulin.
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey, Zebrafish Antibodies
Applications
Conjugation
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Acetyl-alpha-Tubulin (Lys40) (D20G3) Rabbit Monoclonal Antibody detects endogenous levels of α-tubulin only when acetylated at Lys40. This amino acid is not conserved in β-tubulin.
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey, Zebrafish Antibodies
Applications
Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:800 - 1:1600 Immunofluorescence (Immunocytochemistry): 1:800 - 1:1600 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800
Conjugation
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Acetyl-Histone H3 (Lys9) (C5B11) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of histone H3 only when acetylated on Lys9. This antibody does not cross-react with other acetylated histones.
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey, Zebrafish Antibodies
Applications
Conjugation
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Acetyl-Histone H3 (Lys9) (C5B11) Rabbit Monoclonal Antibody detects endogenous levels of histone H3 only when acetylated on Lys9. This antibody does not cross-react with other acetylated histones.
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey, Zebrafish Antibodies
Applications
Western Blotting: 1:1000 Immunoprecipitation: 1:25 Immunohistochemistry (Paraffin): 1:800 Immunofluorescence (Immunocytochemistry): 1:400 Flow Cytometry (Fixed/Permeabilized): 1:200 Chromatin IP: 1:50 Chromatin IP-seq: 1:50 CUT&RUN: 1:50 CUT&Tag: 1:50
Conjugation
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The alpha/beta-Tubulin Antibody detects endogenous levels of both α- and β-tubulin total protein, and does cross-react with both recombinant α- and β-tubulin.
Host
Reactivity
Human, Mouse, Rat, Monkey, Zebrafish Antibodies, Bovine
Applications
Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:100 Immunofluorescence (Immunocytochemistry): 1:50 Flow Cytometry (Fixed/Permeabilized): 1:50
Conjugation
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alpha-Tubulin (11H10) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total α-tubulin protein, and does not cross-react with recombinant β-tubulin.
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey, D. melanogaster, Zebrafish Antibodies, Bovine, Pig
Applications
Conjugation
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