Search results for 504315

Ultra-LEAF Purified anti-mousehuman IL-5 Antibody, IL-5, TRFK5, BioLegend, 504315

ELISA1,3,4or ELISPOT2,6Capture:The purified TRFK5 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated TRFK4 antibody (Cat. No. 504402) as the detection antibody for mouse IL-5, or used in conjunction with biotinylated JES1-5A10 antibody (Cat. No. 501002) as the detection antibody for human IL-5. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.Flow Cytometry:The fluorochrome-labeled TRFK51 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-5-producing cells within mixed cell populations.Neutralization1,9:The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-5 bioactivity (Cat. Nos. 504313-504318).Additional reported applications (for the relevant formats) include:Western blotting, immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections8and paraformaldehyde-fixed, saponin-treated frozen tissue sections7, and immunocytochemistry.Note:For testing human IL-5 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430401 and 430404) are specially developed and recommended. For testing mouse IL-5 in serum, plasma or supernatant, BioLegend's ELISA MAX™ Sets (Cat. Nos. 431201 and 431206) are specially developed and recommended.