Search results for 602752

Purified anti-PIEZO1 Antibody, PIEZO1, W17241A, BioLegend, 602752

When testing this clone for western blot, we observed a considerable loss of signal with lysates that were boiled in sample buffer compared to samples that were heated to 37°C. This is likely due to high temperatures causing aggregation of PIEZO1, which is a multi-pass transmembrane domain protein.This clone was tested for immunocytochemistry on HeLa cells fixed with 4% PFA and permeabilized with either methanol or Triton X-100. Both methods resulted in high non-specific staining.