Search results for 939105

Alexa Fluor® 647 anti-GATA1 Antibody, GATA1, P84F5, BioLegend, 939105

This clone was tested for ICC using 4% PFA-fixed HEL cells permeabilized with either methanol or Triton X-100. While both methods were compatible with robust HEL staining, Triton X-100 permabilization resulted in stronger staining.When using this clone for ICFC, we recommend using the True-Nuclear™ Transcription Factor Buffer Set or the True Phos perm buffer. We do not recommend using the Intracellular Staining Permeabilization Wash Buffer for ICFC testing due to poor GATA1 staining.True-Nuclear™ Transcription Factor Buffer Set allowed for better binding to the epitope when compared to the True Phos perm buffer, there was a twofold difference between their median PE readings.