Search results for APC/Fire
APC/Fire™ 750 anti-human CD11c Antibody, CD11c, 3.9, BioLegend, 301646
Clone 3.9 preferentially binds the activated form of CD11c, is specific for the I domain of CD11c, and is able to partially block the binding of CD11c and ICAM-4. 3.9 binding is divalent cation dependent12. While analyzing blood, it is best to use heparin as the anti-coagulant and not EDTA. Since the ability of clone 3.9 to bind to its target is divalent cation dependent, the usage of EDTA as an anti-coagulant may be detrimental to staining due to its chelating properties.Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections4, and functional assays5,6. The LEAF™purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 301616). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 301632) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Applications
FC -Quality tested
Conjugation
APC/Fire 750 anti-mouse CD31 Antibody anti-CD31 - 390, BioLegend, 102433
Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8Additional reported applications (for the relevant formats) include: immunoprecipitation1,in vitroandin vivoblocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections.Special Note:This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102412).
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
APC/Fire 750 anti-mouse CD31 Antibody anti-CD31 - 390, BioLegend, 102434
Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8Additional reported applications (for the relevant formats) include: immunoprecipitation1,in vitroandin vivoblocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections.Special Note:This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102412).
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
APC/Fire™ 750 anti-human CD166 Antibody, CD166, 3A6, BioLegend, 343909
Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections and immunofluorescence.1
Host
Mouse
Reactivity
Human
Applications
FC -Quality tested
Conjugation
APC/Fire™ 750 anti-human CD166 Antibody, CD166, 3A6, BioLegend, 343910
Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections and immunofluorescence.1
Host
Mouse
Reactivity
Human
Applications
FC -Quality tested
Conjugation
APC/Fire™ 750 anti-mouse CD25 Antibody CD25, 3C7, BioLegend, 101921
Additional reported applications (for the relevant formats) include:in vitroblocking of IL-2 binding to low- and high-affinity receptors1,2, and immunohistochemical staining of acetone-fixed frozen sections. 3C7 antibody recognizes different epitope of PC61 antibody (Cat. No. 102002). The Ultra-LEAF™Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No.101925 and 101926).
Host
Rat
Reactivity
Mouse
Applications
FC -Quality tested
Conjugation
APC/Fire™ 750 anti-mouse CD25 Antibody CD25, 3C7, BioLegend, 101922
Additional reported applications (for the relevant formats) include:in vitroblocking of IL-2 binding to low- and high-affinity receptors1,2, and immunohistochemical staining of acetone-fixed frozen sections. 3C7 antibody recognizes different epitope of PC61 antibody (Cat. No. 102002). The Ultra-LEAF™Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No.101925 and 101926).
Host
Rat
Reactivity
Mouse
Applications
FC -Quality tested
Conjugation
APC/Fire 750 anti-human CD16 Antibody anti-CD16 - 3G8, BioLegend, 302059
The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the FcγRIIa and FcγRIIIb receptors causing neutrophil activation and aggregation18.Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile.Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6, immunoprecipitation3, stimulation of NK cell proliferation4, blocking of phagocytosis5, and blocking of immunoglobulin binding to FcγRIII7,8. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Applications
FC - Quality tested
Conjugation
APC/Fire 750 anti-human CD16 Antibody anti-CD16 - 3G8, BioLegend, 302060
The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the FcγRIIa and FcγRIIIb receptors causing neutrophil activation and aggregation18.Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile.Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6, immunoprecipitation3, stimulation of NK cell proliferation4, blocking of phagocytosis5, and blocking of immunoglobulin binding to FcγRIII7,8. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Applications
FC - Quality tested
Conjugation
APC/Fire™ 810 anti-human CD16 Antibody, CD16, 3G8, BioLegend, 302073
The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the FcγRIIa and FcγRIIIb receptors causing neutrophil activation and aggregation18.Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile.Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6, immunoprecipitation3, stimulation of NK cell proliferation4, blocking of phagocytosis5, and blocking of immunoglobulin binding to FcγRIII7,8. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Applications
FC - Quality tested
Conjugation
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