Search results for TLR4 antibody
PE anti-mouse CD284 TLR4 Antibody anti-CD284 - SA15-21, BioLegend, 145404
Anti-mouse TLR4 clones SA15-21 and MTS510 recognize distinct epitopes and don’t cross block each other.1Clone MTS510 binds to an epitope of TLR4/MD-2 that is lost after LPS stimulation.1Unlike MTS510, the binding of SA15-21 is not interrupted by LPS stimulation1. Clone SA15-21 is capable of recognizing TLR4 individually and when it is complexed with MD-2.Additional reported applications (for the relevant formats) include: immunoprecipitation1and inhibition of LPS-induced hepatocyte apoptosis2. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for functional assays (contact ourcustom solutions team).
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
APC anti-mouse CD284 TLR4 Antibody anti-CD284 - SA15-21, BioLegend, 145405
Anti-mouse TLR4 clones SA15-21 and MTS510 recognize distinct epitopes and don’t cross block each other.1Clone MTS510 binds to an epitope of TLR4/MD-2 that is lost after LPS stimulation.1Unlike MTS510, the binding of SA15-21 is not interrupted by LPS stimulation1. Clone SA15-21 is capable of recognizing TLR4 individually and when it is complexed with MD-2.Additional reported applications (for the relevant formats) include: immunoprecipitation1and inhibition of LPS-induced hepatocyte apoptosis2. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for functional assays (contact ourcustom solutions team).
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
APC anti-mouse CD284 TLR4 Antibody anti-CD284 - SA15-21, BioLegend, 145406
Anti-mouse TLR4 clones SA15-21 and MTS510 recognize distinct epitopes and don’t cross block each other.1Clone MTS510 binds to an epitope of TLR4/MD-2 that is lost after LPS stimulation.1Unlike MTS510, the binding of SA15-21 is not interrupted by LPS stimulation1. Clone SA15-21 is capable of recognizing TLR4 individually and when it is complexed with MD-2.Additional reported applications (for the relevant formats) include: immunoprecipitation1and inhibition of LPS-induced hepatocyte apoptosis2. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for functional assays (contact ourcustom solutions team).
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
Biotin anti-mouse CD284 TLR4 Antibody anti-CD284 - SA15-21, BioLegend, 145409
Anti-mouse TLR4 clones SA15-21 and MTS510 recognize distinct epitopes and don’t cross block each other.1Clone MTS510 binds to an epitope of TLR4/MD-2 that is lost after LPS stimulation.1Unlike MTS510, the binding of SA15-21 is not interrupted by LPS stimulation1. Clone SA15-21 is capable of recognizing TLR4 individually and when it is complexed with MD-2.Additional reported applications (for the relevant formats) include: immunoprecipitation1and inhibition of LPS-induced hepatocyte apoptosis2. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for functional assays (contact ourcustom solutions team).
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
TotalSeq™-B 0405 anti-human CD284 (TLR4) Antibody, CD284, HTA125, BioLegend, 312821
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4, immunofluorescence microscopy6, Western blotting10, andin vitroblocking of LPS-induced cytokine production2,3,7,9. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 312806) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 312804) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 312813 & 312814).
Host
Mouse
Reactivity
Human
Applications
PG - Quality tested
Conjugation
Brilliant Violet 421 anti-human CD284 TLR4 Antibody anti-CD284 - HTA125, BioLegend, 312811
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4, immunofluorescence microscopy6, Western blotting10, andin vitroblocking of LPS-induced cytokine production2,3,7,9. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 312806) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 312804) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 312813 & 312814).
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Ultra-LEAF Purified anti-human CD284 TLR4 Antibody anti-CD284 - HTA125, BioLegend, 312813
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4, immunofluorescence microscopy6, Western blotting10, andin vitroblocking of LPS-induced cytokine production2,3,7,9. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 312806) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 312804) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 312813 & 312814).
Host
Mouse
Reactivity
Human
Applications
FC -Quality testedIHC-F, ICC, WB, Block -Reported in the literature, not verified in house
Conjugation
Ultra-LEAF Purified anti-human CD284 TLR4 Antibody anti-CD284 - HTA125, BioLegend, 312814
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4, immunofluorescence microscopy6, Western blotting10, andin vitroblocking of LPS-induced cytokine production2,3,7,9. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 312806) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 312804) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 312813 & 312814).
Host
Mouse
Reactivity
Human
Applications
FC -Quality testedIHC-F, ICC, WB, Block -Reported in the literature, not verified in house
Conjugation
PE/Cyanine7 anti-mouse CD284 TLR4 Antibody anti-CD284 - SA15-21, BioLegend, 145407
Anti-mouse TLR4 clones SA15-21 and MTS510 recognize distinct epitopes and don’t cross block each other.1Clone MTS510 binds to an epitope of TLR4/MD-2 that is lost after LPS stimulation.1Unlike MTS510, the binding of SA15-21 is not interrupted by LPS stimulation1. Clone SA15-21 is capable of recognizing TLR4 individually and when it is complexed with MD-2.Additional reported applications (for the relevant formats) include: immunoprecipitation1and inhibition of LPS-induced hepatocyte apoptosis2. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for functional assays (contact ourcustom solutions team).
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
PE/Cyanine7 anti-mouse CD284 TLR4 Antibody anti-CD284 - SA15-21, BioLegend, 145408
Anti-mouse TLR4 clones SA15-21 and MTS510 recognize distinct epitopes and don’t cross block each other.1Clone MTS510 binds to an epitope of TLR4/MD-2 that is lost after LPS stimulation.1Unlike MTS510, the binding of SA15-21 is not interrupted by LPS stimulation1. Clone SA15-21 is capable of recognizing TLR4 individually and when it is complexed with MD-2.Additional reported applications (for the relevant formats) include: immunoprecipitation1and inhibition of LPS-induced hepatocyte apoptosis2. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for functional assays (contact ourcustom solutions team).
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
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