Search results for 387
Spark UV™ 387 anti-mouse CD86 (Flexi-Fluor™) Antibody, CD86, GL-1, BioLegend, 285493
The GL-1 antibody can block the mixed lymphocyte reactionin vitroand has been shown to inhibit the priming of cytotoxic T lymphocytesin vivo(along with antibodies against B7-1). Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen sections2,6, immunofluorescence microscopy, andin vivoandin vitroblocking of T cell responses1-6. GL-1 is not suitable for immunohistochemical staining of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105051-105056).
Host
Rat
Reactivity
Mouse
Applications
FC
Conjugation
Spark UV™ 387 anti-human CD33 (Flexi-Fluor™) Antibody, CD33, HIM3-4, BioLegend, 285513
Additional reported applications include: immunofluorescence2.
Host
Mouse
Reactivity
Human
Applications
FC
Conjugation
Spark UV™ 387 anti-mouse/human CD44 (Flexi-Fluor™) Antibody, CD44, IM7, BioLegend, 285498
Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD4417,18that is located between amino acids 145 and 18620. This clone has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3,in vivoinhibition of DTH4,5, and spatial biology (IBEX)23,24. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103046, 103065 - 103069).Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.
Host
Rat
Reactivity
Mouse, Human
Applications
FC
Conjugation
Spark UV™ 387 anti-mouse CD62L (Flexi-Fluor™) Antibody, CD62L, MEL-14, BioLegend, 285499
Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, complement-dependent cytotoxicity4,in vivoandin vitroblocking of adhesion1-3,5, and immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections6. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104457-104462).
Host
Rat
Reactivity
Mouse
Applications
FC
Conjugation
Spark UV™ 387 anti-mouse/human CD45R/B220 Antibody, CD45R/B220, RA3-6B2, BioLegend, 103295
Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1,in vitroandin vivomodulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.
Host
Rat
Reactivity
Mouse, Human
Applications
FC - Quality tested
Conjugation
Spark UV™ 387 anti-mouse/human CD45R/B220 Antibody, CD45R/B220, RA3-6B2, BioLegend, 103296
Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1,in vitroandin vivomodulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.
Host
Rat
Reactivity
Mouse, Human
Applications
FC - Quality tested
Conjugation
Spark UV™ 387 anti-human CD8a (Flexi-Fluor™) Antibody, CD8a, RPA-T8, BioLegend, 285370
The RPA-T8 antibody does not block the binding of HIT8a antibody to CD8a. Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed frozen sections3and costimulation of T cell responses4. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 301073 & 301074).
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Applications
FC
Conjugation
Spark UV™ 387 anti-human CD117 (c-kit) (Flexi-Fluor™) Antibody, CD117, 104D2, BioLegend, 285506
The 104D2 antibody does not block binding of c-Kit ligand. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy1, and spatial biology (IBEX)4,5.
Host
Mouse
Reactivity
Human
Applications
FC
Conjugation
Spark UV™ 387 anti-human HLA-A,B,C Antibody, HLA-A,B,C, W6/32, BioLegend, 311461
Clone W6/32 recognizes residues in the N terminus of the human ß2-microglobulin molecule21.Additional reported applications (for the relevant formats) include: immunoprecipitaton2, Western blotting (non-reducing)3, immunohistochemical staining of acetone-fixed frozen tissue sections4,5, blocking6,7, inhibition of NK cell-mediated lysis10, and activation8,9. Clone W6/32 has been reported not to be suitable for immunohistochemistry on paraffin sections17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 311428) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Applications
FC - Quality tested
Conjugation
Spark UV™ 387 anti-human HLA-A,B,C Antibody, HLA-A,B,C, W6/32, BioLegend, 311462
Clone W6/32 recognizes residues in the N terminus of the human ß2-microglobulin molecule21.Additional reported applications (for the relevant formats) include: immunoprecipitaton2, Western blotting (non-reducing)3, immunohistochemical staining of acetone-fixed frozen tissue sections4,5, blocking6,7, inhibition of NK cell-mediated lysis10, and activation8,9. Clone W6/32 has been reported not to be suitable for immunohistochemistry on paraffin sections17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 311428) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Applications
FC - Quality tested
Conjugation
Please provide the required information below so that we can quickly source your products.