Search results for CCL2
Ultra-LEAF Purified anti-mouserathuman MCP-1 Antibody, CCL2, 2H5, BioLegend, 505915
ELISA or ELISPOT Capture1:The Ultra-LEAF™ 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4E2/MCP antibody (Cat. No. 506002) as the detecting antibody for the detection of mouse MCP-1. The purified 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 5D3-F7 antibody (Cat. No. 502609) as the detecting antibody for the detection of human MCP-1.Flow Cytometry2:The fluorochrome-labeled 2H5 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify MCP-1 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.Neutralization1,4,6:The Ultra-LEAF™ purified antibody (Cat. Nos. 505911 & 505912) is recommended for neutralization.Additional reported applications (for the relevant formats) include:Western blotting3, immunohistochemistry of paraformaldehyde-fixed, saponin-treated frozen tissue sections5, and acetone-fixed frozen tissue sections7.
Host
Hamster
Reactivity
Mouse, Rat, Human
Applications
ELISA Capture -Quality testedELISPOT Capture, Neut, WB, IHC -Reported in the literature, not verified in house
Conjugation
Ultra-LEAF Purified anti-mouserathuman MCP-1 Antibody, CCL2, 2H5, BioLegend, 505916
ELISA or ELISPOT Capture1:The Ultra-LEAF™ 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4E2/MCP antibody (Cat. No. 506002) as the detecting antibody for the detection of mouse MCP-1. The purified 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 5D3-F7 antibody (Cat. No. 502609) as the detecting antibody for the detection of human MCP-1.Flow Cytometry2:The fluorochrome-labeled 2H5 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify MCP-1 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.Neutralization1,4,6:The Ultra-LEAF™ purified antibody (Cat. Nos. 505911 & 505912) is recommended for neutralization.Additional reported applications (for the relevant formats) include:Western blotting3, immunohistochemistry of paraformaldehyde-fixed, saponin-treated frozen tissue sections5, and acetone-fixed frozen tissue sections7.
Host
Hamster
Reactivity
Mouse, Rat, Human
Applications
ELISA Capture -Quality testedELISPOT Capture, Neut, WB, IHC -Reported in the literature, not verified in house
Conjugation
Biotin anti-mouse MCP-1 Antibody anti-CCL2 - 4E2/MCP, BioLegend, 506002
ELISA or ELISPOT Capture:The purified 4E2/MCP antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 2H5 antibody (Cat. No. 505908) as the detecting antibody for the detection of mouse MCP-1. The LEAF™ purified antibody is suggested for ELISPOT capture.Additional reported applications (for the relevant formats) include:Western blotting1. The 4E2/MCP is a non-neutralizing antibody1.
Host
Hamster
Reactivity
Mouse
Applications
ELISA Detection, ELISPOT Detection
Conjugation
Purified anti-mouse MCP-1 Antibody anti-CCL2 - 4E2/MCP, BioLegend, 506004
ELISA or ELISPOT Capture:The purified 4E2/MCP antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 2H5 antibody (Cat. No. 505908) as the detecting antibody for the detection of mouse MCP-1. The LEAF™ purified antibody is suggested for ELISPOT capture.Additional reported applications (for the relevant formats) include:Western blotting1. The 4E2/MCP is a non-neutralizing antibody1.
Host
Hamster
Reactivity
Mouse
Applications
ELISA Capture - Quality testedWB - Reported in the literature, not verified in house
Conjugation
Biotin anti-human MCP-1 Antibody anti-CCL2 - 5D3-F7, BioLegend, 502608
ELISA or ELISPOT Detection1:The biotinylated 5D3-F7 antibody is useful as the detection antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified 2H5 antibody (Cat. No. 505902/505906) as the capture antibody.ELISA or ELISPOT Capture:The purified 5D3-F7 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 2H5 antibody (Cat. No. 505908) as the detection antibody. The LEAF™ purified antibody (Cat. No. 502607) is suggested for ELISPOT capture.Additional reported applications (for the relevant formats) include: intracellular flow cytometry2, immunoprecipitation1,3, Western blotting1, and immunohistochemical staining1.
Host
Mouse
Reactivity
Human
Applications
ELISA Detection, ELISPOT Detection, ICFC
Conjugation
Biotin anti-human MCP-1 Antibody anti-CCL2 - 5D3-F7, BioLegend, 502609
ELISA or ELISPOT Detection1:The biotinylated 5D3-F7 antibody is useful as the detection antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified 2H5 antibody (Cat. No. 505902/505906) as the capture antibody.ELISA or ELISPOT Capture:The purified 5D3-F7 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 2H5 antibody (Cat. No. 505908) as the detection antibody. The LEAF™ purified antibody (Cat. No. 502607) is suggested for ELISPOT capture.Additional reported applications (for the relevant formats) include: intracellular flow cytometry2, immunoprecipitation1,3, Western blotting1, and immunohistochemical staining1.
Host
Mouse
Reactivity
Human
Applications
ELISA Detection, ELISPOT Detection, ICFC
Conjugation
PE anti-human MCP-1 Antibody anti-CCL2 - 5D3-F7, BioLegend, 502604
ELISA or ELISPOT Detection1:The biotinylated 5D3-F7 antibody is useful as the detection antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified 2H5 antibody (Cat. No. 505902/505906) as the capture antibody.ELISA or ELISPOT Capture:The purified 5D3-F7 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 2H5 antibody (Cat. No. 505908) as the detection antibody. The LEAF™ purified antibody (Cat. No. 502607) is suggested for ELISPOT capture.Additional reported applications (for the relevant formats) include: intracellular flow cytometry2, immunoprecipitation1,3, Western blotting1, and immunohistochemical staining1.
Host
Mouse
Reactivity
Human
Applications
ICFC - Quality tested
Conjugation
Purified anti-human MCP-1 Antibody anti-CCL2 - 5D3-F7, BioLegend, 502601
ELISA or ELISPOT Detection1:The biotinylated 5D3-F7 antibody is useful as the detection antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified 2H5 antibody (Cat. No. 505902/505906) as the capture antibody.ELISA or ELISPOT Capture:The purified 5D3-F7 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 2H5 antibody (Cat. No. 505908) as the detection antibody. The LEAF™ purified antibody (Cat. No. 502607) is suggested for ELISPOT capture.Additional reported applications (for the relevant formats) include: intracellular flow cytometry2, immunoprecipitation1,3, Western blotting1, and immunohistochemical staining1.
Host
Mouse
Reactivity
Human
Applications
ELISA Capture - Quality testedWB - VerifiedELISA & ELISPOT Detection, ICFC, IHC, IP - Reported in the literature, not verified in house
Conjugation
Purified anti-human MCP-1 Antibody anti-CCL2 - 5D3-F7, BioLegend, 502602
ELISA or ELISPOT Detection1:The biotinylated 5D3-F7 antibody is useful as the detection antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified 2H5 antibody (Cat. No. 505902/505906) as the capture antibody.ELISA or ELISPOT Capture:The purified 5D3-F7 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 2H5 antibody (Cat. No. 505908) as the detection antibody. The LEAF™ purified antibody (Cat. No. 502607) is suggested for ELISPOT capture.Additional reported applications (for the relevant formats) include: intracellular flow cytometry2, immunoprecipitation1,3, Western blotting1, and immunohistochemical staining1.
Host
Mouse
Reactivity
Human
Applications
ELISA Capture - Quality testedWB - VerifiedELISA & ELISPOT Detection, ICFC, IHC, IP - Reported in the literature, not verified in house
Conjugation
Purified anti-mouse/rat/human MCP-1 Antibody anti-CCL2 - 2H5, BioLegend, 505901
ELISA or ELISPOT Capture1:The Ultra-LEAF™ 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4E2/MCP antibody (Cat. No. 506002) as the detecting antibody for the detection of mouse MCP-1. The purified 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 5D3-F7 antibody (Cat. No. 502609) as the detecting antibody for the detection of human MCP-1.Flow Cytometry2:The fluorochrome-labeled 2H5 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify MCP-1 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.Neutralization1,4,6:The Ultra-LEAF™ purified antibody (Cat. Nos. 505911 & 505912) is recommended for neutralization.Additional reported applications (for the relevant formats) include:Western blotting3, immunohistochemistry of paraformaldehyde-fixed, saponin-treated frozen tissue sections5, and acetone-fixed frozen tissue sections7.
Host
Hamster
Reactivity
Mouse, Rat, Human
Applications
ELISA Capture - Quality testedWB, IHC - Reported in the literature, not verified in house
Conjugation
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