Search results for 718

Spark Red™ 718 anti-human CD26 (Flexi-Fluor™) Antibody, CD26, BA5b, BioLegend, 285061

Spark Red™ 718 anti-human CD21 (Flexi-Fluor™) Antibody, CD21, Bu32, BioLegend, 285064

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4.

Spark Red™ 718 anti-human/mouse/rat CD278 (ICOS) Antibody, CD278, C398.4A, BioLegend, 313563

The C398.4A antibody is useful for flow cytometric analysis and is able to costimulate T cell activation and proliferation. Additional reported applications (for the relevant formats) include: immunoprecipitation1,in vitrocostimulation of T cell activation1,3,4, and spatial biology (IBEX)5,6. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 313512).

Spark Red™ 718 anti-human/mouse/rat CD278 (ICOS) Antibody, CD278, C398.4A, BioLegend, 313564

The C398.4A antibody is useful for flow cytometric analysis and is able to costimulate T cell activation and proliferation. Additional reported applications (for the relevant formats) include: immunoprecipitation1,in vitrocostimulation of T cell activation1,3,4, and spatial biology (IBEX)5,6. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 313512).

Spark Red™ 718 anti-human CD71 (Flexi-Fluor™) Antibody, CD71, CY1G4, BioLegend, 285067

Spark Red™ 718 anti-mouse CD4 (Flexi-Fluor™) Antibody, CD4, GK1.5, BioLegend, 285218

Additional reported applications (for the relevant formats) include: blocking of CD4+T cell activation1,4,11, thymocyte costimulation3,in vitroandin vivodepletion2,5-8, blocking of egg-sperm cell adhesion1,4, immunohistochemical staining of acetone-fixed frozen sections9,10, immunoprecipitation1,2, and spatial biology (IBEX)12,13. The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Spark Red™ 718 anti-human CD11a (Flexi-Fluor™) Antibody, CD11a, HI111, BioLegend, 285076

Clone HI111 epitope maps to the top region of the I domain that is close to the putative ligand-binding site surrounding the MIDAS (metal ion-dependent adhesion site). HI111 is specific for the closed confirmation of the integrin8. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, Western blotting2, and blocking of cell-cell interaction and inhibition the binding of ICAM-14. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301233 & 301234).

Spark Red™ 718 anti-human CD235ab (Flexi-Fluor™) Antibody, CD235ab, HIR2, BioLegend, 285343

Spark Red™ 718 anti-human CD11b (Flexi-Fluor™) Antibody, CD11b, ICRF44, BioLegend, 285077

The ICRF44 antibody inhibits heterotypic adhesion of granulocytes in response to fMLP. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, immunofluorescence microscopy5, stimulation of monocytes3, blocking of heterotypic PMN aggregation8, and blocking of granulocyte activation12. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 301361 & 301362).

Spark Red™ 718 anti-mouse IL-2 Antibody, IL-2, JES6-5H4, BioLegend, 503851

ELISA Detection1-3or ELISPOT Detection4-6: The biotinylated JES6-5H4 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified JES6-1A12 antibody (Cat. Nos. 503701 & 503702) as capture antibody and recombinant mouse IL-2 (Cat. No. 575409) as the standard.Flow Cytometry8-10: The fluorochrome-labeled JES6-5H4 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-2 -producing cells within mixed cell populations.Neutralization1,7: The Ultra-LEAF™ purified antibody (Endotoxinin vivoandin vitro(Cat. No. 503845-503850)) is recommended for neutralization.Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections2,in vivocapture7, and immunocytochemistry.Note:For testing mouse IL-2 in serum, plasma or supernatant, BioLegend's ELISA MAX™ Sets (Cat. No. 431001 & 431004) are specially developed and recommended.