Search results for 718

Spark Red™ 718 anti-mouse IL-2 Antibody, IL-2, JES6-5H4, BioLegend, 503852
ELISA Detection1-3or ELISPOT Detection4-6: The biotinylated JES6-5H4 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified JES6-1A12 antibody (Cat. Nos. 503701 & 503702) as capture antibody and recombinant mouse IL-2 (Cat. No. 575409) as the standard.Flow Cytometry8-10: The fluorochrome-labeled JES6-5H4 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-2 -producing cells within mixed cell populations.Neutralization1,7: The Ultra-LEAF™ purified antibody (Endotoxinin vivoandin vitro(Cat. No. 503845-503850)) is recommended for neutralization.Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections2,in vivocapture7, and immunocytochemistry.Note:For testing mouse IL-2 in serum, plasma or supernatant, BioLegend's ELISA MAX™ Sets (Cat. No. 431001 & 431004) are specially developed and recommended.
Host
Rat
Reactivity
Mouse
Applications
ICFC - Quality tested
Conjugation

Spark Red™ 718 anti-human TCR Cβ1 Antibody, TCR Cβ1, JOVI.1, BioLegend, 383521
Clone JOVI.1 can interfere with the binding of clone IP26 on target cells.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation

Spark Red™ 718 anti-human TCR Cβ1 Antibody, TCR Cβ1, JOVI.1, BioLegend, 383522
Clone JOVI.1 can interfere with the binding of clone IP26 on target cells.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation

Spark Red™ 718 anti-mouse CD3ε (Flexi-Fluor™) Antibody, CD3ε, KT3.1.1, BioLegend, 285083
Host
Rat
Reactivity
Mouse
Applications
FC
Conjugation

Spark Red™ 718 anti-human CD33 (Flexi-Fluor™)Antibody, CD33, P67.6, BioLegend, 285100
Host
Mouse
Reactivity
Human
Applications
FC
Conjugation

Spark Red™ 718 anti-mouse CX3CR1 (Flexi-Fluor™) Antibody, CX3CR1, SA011F11, BioLegend, 285107
View more applications data for a different format of this clone in ourScientific Poster Library.
Host
Mouse
Reactivity
Mouse
Applications
FC
Conjugation

Spark Red™ 718 anti-human CD5 (Flexi-Fluor™) Antibody, CD5, UCHT2, BioLegend, 285115
Additional reported applications (for the relevant formats) include: Western blotting2and immunohistochemical staining of acetone-fixed frozen sections2,5.
Host
Mouse
Reactivity
Human
Applications
FC
Conjugation

Spark Red™ 718 anti-human CD31 (Flexi-Fluor™) Antibody, CD31, WM59, BioLegend, 285119
Clone WM59 has been reported to recognize the D2 extracellular portion of CD31.Additional reported applications (for the relevant formats) include: immunofluorescence microscopy2, immunohistochemical staining of acetone-fixed frozen tissue sections8, blocking of platelet aggregation3, and spatial biology (IBEX)11,12. Clone WM59 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 303143 & 303144).The purified WM59 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with biotin anti-human CD31 antibody (Cat. No. 536604) antibody as the detection antibody.
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Applications
FC
Conjugation

Spark Red™ 718 anti-human CD159a (NKG2A) (Flexi-Fluor™) Antibody, CD159a, S19004C, BioLegend, 285106
Clone S19004C does not cross-react with mouse CD159a.
Host
Mouse
Reactivity
Human
Applications
FC
Conjugation

Spark Red™ 718 anti-human CD366 (Tim-3) Antibody, CD366 (Tim-3), A18087E, BioLegend, 364817
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
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