Search results for 650
Brilliant Violet 650 anti-mouse IFN-gamma Antibody anti-IFN-gamma - XMG1.2, BioLegend, 505831
ELISA1-4,11,14or ELISPOT5Detection:The biotinylated XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified R4-6A2 antibody (Cat. No. 505702/505706) as the capture antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard.ELISA or ELISPOT Capture:The purified XMG1.2 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as the detection antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture (Cat. No. 505812).Flow Cytometry7,8,12,13,16:The fluorochrome-labeled XMG1.2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ-producing cells within mixed cell populations.Neutralization1-3,9,10:The XMG1.2 antibody can neutralize the bioactivity of natural or recombinant IFN-γ. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IFN-γ bioactivityin vivoandin vitro(Cat. No. 505812). Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 505834) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).Additional reported applications (for the relevant formats) include:Western blotting, immunohistochemical staining of frozen tissue sections6,22,23, and immunocytochemistry.Note:For testing mouse IFN-γ in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430801 to 430806) are specially developed and recommended.
Host
Rat
Reactivity
Mouse
Applications
ICFC -Quality tested
Conjugation
Brilliant Violet 650 anti-mouse IFN-gamma Antibody anti-IFN-gamma - XMG1.2, BioLegend, 505832
ELISA1-4,11,14or ELISPOT5Detection:The biotinylated XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified R4-6A2 antibody (Cat. No. 505702/505706) as the capture antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard.ELISA or ELISPOT Capture:The purified XMG1.2 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as the detection antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture (Cat. No. 505812).Flow Cytometry7,8,12,13,16:The fluorochrome-labeled XMG1.2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ-producing cells within mixed cell populations.Neutralization1-3,9,10:The XMG1.2 antibody can neutralize the bioactivity of natural or recombinant IFN-γ. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IFN-γ bioactivityin vivoandin vitro(Cat. No. 505812). Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 505834) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).Additional reported applications (for the relevant formats) include:Western blotting, immunohistochemical staining of frozen tissue sections6,22,23, and immunocytochemistry.Note:For testing mouse IFN-γ in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430801 to 430806) are specially developed and recommended.
Host
Rat
Reactivity
Mouse
Applications
ICFC -Quality tested
Conjugation
Brilliant Violet 650 anti-human CD223 LAG-3 Antibody anti-CD223 - 11C3C65, BioLegend, 369315
The staining of clone 11C3C65 cannot be blocked by clone 7H2C65, which is another anti-human CD223 (LAG-3) antibody.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Brilliant Violet 650 anti-human CD223 LAG-3 Antibody anti-CD223 - 11C3C65, BioLegend, 369316
The staining of clone 11C3C65 cannot be blocked by clone 7H2C65, which is another anti-human CD223 (LAG-3) antibody.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
![DyLight® 650 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] Brand Logo](https://file.buyantibody.com/public/brand/20260608/Abcam.jpg "Click to View DyLight® 650 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] Product Detail")
DyLight® 650 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2], Abcam, AB117492
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
Host
Mouse
Reactivity
Applications
ICC/IF
Conjugation
DyLight® 650
Brilliant Violet 650 anti-mouse CD138 Syndecan-1 Antibody anti-CD138 - 281-2, BioLegend, 142517
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue3and formalin-fixed paraffin embedded tissue4and immunofluorescent staining2,3.
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
Brilliant Violet 650 anti-mouse CD138 Syndecan-1 Antibody anti-CD138 - 281-2, BioLegend, 142518
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue3and formalin-fixed paraffin embedded tissue4and immunofluorescent staining2,3.
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
Brilliant Violet 650™ anti-mouse CD279 (PD-1) Antibody, CD279, 29F.1A12, BioLegend, 135243
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue3,in vivoblocking of PD-1 binding to its ligands2,3, and spatial biology (IBEX)5,6.
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
Brilliant Violet 650 anti-human CD137 4-1BB Antibody anti-CD137 - 4B4-1, BioLegend, 309827
Additional reported applications (for the relevant formats) include: immunoprecipitation1,4, inhibition of cytokine production2,3, and ELISA. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 309804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by Streptavidin-PE (Cat. No. 405204)).
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Brilliant Violet 650 anti-human CD137 4-1BB Antibody anti-CD137 - 4B4-1, BioLegend, 309828
Additional reported applications (for the relevant formats) include: immunoprecipitation1,4, inhibition of cytokine production2,3, and ELISA. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 309804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by Streptavidin-PE (Cat. No. 405204)).
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
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