Search results for 421
Brilliant Violet 421 anti-T-bet Antibody anti-T-bet - 4B10, BioLegend, 644815
Additional reported applications (for the relevant formats) include: immunoprecipitation2and immunofluorescence microscopy3.NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No.424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).
Host
Mouse
Reactivity
Human, Mouse
Applications
ICFC -Quality tested
Conjugation
Brilliant Violet 421 anti-T-bet Antibody anti-T-bet - 4B10, BioLegend, 644832
Additional reported applications (for the relevant formats) include: immunoprecipitation2and immunofluorescence microscopy3.NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No.424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).
Host
Mouse
Reactivity
Human, Mouse
Applications
ICFC -Quality tested
Conjugation
Brilliant Violet 421 anti-T-bet Antibody anti-T-bet - 4B10, BioLegend, 644816
Additional reported applications (for the relevant formats) include: immunoprecipitation2and immunofluorescence microscopy3.NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No.424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).
Host
Mouse
Reactivity
Human, Mouse
Applications
ICFC -Quality tested
Conjugation
Brilliant Violet 421 anti-mouse CD197 CCR7 Antibody anti-CD197 - 4B12, BioLegend, 120119
The 4B12 antibody does not inhibit binding of ligand to receptor. Additional reported applications (for the relevant formats) include: immunoprecipitation. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302) is recommended prior to immunofluorescent staining.Staining with clone 4B12 is recommended at 37°C(see supplemental data of PE staining at differing temperatures).
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
Brilliant Violet 421 anti-mouse CD197 CCR7 Antibody anti-CD197 - 4B12, BioLegend, 120120
The 4B12 antibody does not inhibit binding of ligand to receptor. Additional reported applications (for the relevant formats) include: immunoprecipitation. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302) is recommended prior to immunofluorescent staining.Staining with clone 4B12 is recommended at 37°C(see supplemental data of PE staining at differing temperatures).
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
Brilliant Violet 421 anti-mouse CD8a Antibody anti-CD8a - 53-6.7, BioLegend, 100737
Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3,in vivoandin vitrocell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, immunohistochemical staining5,6of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).
Host
Rat
Reactivity
Mouse
Applications
FC - Quality testedIHC - VerifiedSB - Reported in the literature, not verified in house
Conjugation
Brilliant Violet 421 anti-mouse CD8a Antibody anti-CD8a - 53-6.7, BioLegend, 100753
Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3,in vivoandin vitrocell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, immunohistochemical staining5,6of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).
Host
Rat
Reactivity
Mouse
Applications
FC - Quality testedIHC - VerifiedSB - Reported in the literature, not verified in house
Conjugation
Brilliant Violet 421 anti-mouse CD8a Antibody anti-CD8a - 53-6.7, BioLegend, 100738
Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3,in vivoandin vitrocell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, immunohistochemical staining5,6of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).
Host
Rat
Reactivity
Mouse
Applications
FC - Quality testedIHC - VerifiedSB - Reported in the literature, not verified in house
Conjugation
Brilliant Violet 421 anti-mouse CD5 Antibody anti-CD5 - 53-7.3, BioLegend, 100617
Additional reported applications (for the relevant formats) include: immunoprecipitation1, and immunohistochemistry2of acetone-fixed frozen tissue sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections.
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
Brilliant Violet 421 anti-mouse CD5 Antibody anti-CD5 - 53-7.3, BioLegend, 100629
Additional reported applications (for the relevant formats) include: immunoprecipitation1, and immunohistochemistry2of acetone-fixed frozen tissue sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections.
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
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