Search results for IFN-gamma
IFN-gamma Primary Antibody, ProMab, 20009
Interferon-? (IFN-?) is a pro-inflammatory cytokine that is central in host resistance to infection. It is mainly produced by natural killer cells and CD4+ and CD8+ T cells, its receptors are found on nearly all cells, where it activates diverse responses that enable potential host cells to prevent invasive infection by bacteria, parasites and viruses. Takayanagi et al. (2000) demonstrated that IFN-? strongly suppresses osteoclastogenesis by interfering with the RANKL (602642)-RANK (603499) signaling pathway. Tsubota et al. (1999) reported that this upregulation in Sjogren syndrome patients may be controlled by interferon-gamma through the activation of transcription factor NFKB
Host
Reactivity
Applications
Conjugation
IFN-gamma Primary Antibody, ProMab, 20160
IFN-gamma (interferon, gamma) is an antiviral and antiparasitic agent produced by CD4+/CD8+ lymphocytes and natural killer cells that undergo activation by antigens, mitogens or alloantigens. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses, including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. The active form of IFN-G is a homodimer with each subunit containing six helices. The dimeric structure of human IFN-G is stabilized by non-covalent interactions through the interface of the helices. IFN-G translated precursor is 166 amino acids, including the 23 amino acid secretory sequence. It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Multiple forms exist due to variable glycosylation and under non-denaturing conditions due to dimers and tetramers.
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Reactivity
Applications
Conjugation
Purified anti-rat IFN-gamma Antibody anti-IFN-gamma - DB-1, BioLegend, 507801
ELISA Capture1or ELISPOT Capture2:The purified DB-1 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated poly5188 antibody (Cat. No. 518803) as the detecting antibody and recombinant IFN-? (Cat. No. 565701) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture.Flow Cytometry5:The fluorochrome-labeled DB-1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-?-producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.Neutralization3,4:The LEAF™ purified antibody (Endotoxin in vivo andin vitro(Cat. No. 507808).Additional reported applications (for the relevant formats) include:Western blotting1, and immunohistochemistry2of paraformaldehyde-fixed, saponin-treated frozen tissue sections.
Host
Mouse
Reactivity
Mouse, Rat
Applications
ELISA Capture -Quality testedICFC, IHC-F, WB -Reported in the literature, not verified in house
Conjugation
Purified anti-rat IFN-gamma Antibody anti-IFN-gamma - DB-1, BioLegend, 507802
ELISA Capture1or ELISPOT Capture2:The purified DB-1 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated poly5188 antibody (Cat. No. 518803) as the detecting antibody and recombinant IFN-? (Cat. No. 565701) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture.Flow Cytometry5:The fluorochrome-labeled DB-1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-?-producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.Neutralization3,4:The LEAF™ purified antibody (Endotoxin in vivo andin vitro(Cat. No. 507808).Additional reported applications (for the relevant formats) include:Western blotting1, and immunohistochemistry2of paraformaldehyde-fixed, saponin-treated frozen tissue sections.
Host
Mouse
Reactivity
Mouse, Rat
Applications
ELISA Capture -Quality testedICFC, IHC-F, WB -Reported in the literature, not verified in house
Conjugation
FITC anti-rat IFN-gamma Antibody anti-IFN-gamma - DB-1, BioLegend, 507804
ELISA Capture1or ELISPOT Capture2:The purified DB-1 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated poly5109 antibody (Cat. No. 510901) as the detecting antibody and recombinant IFN-γ (Cat. No. 565701) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture.Flow Cytometry5:The fluorochrome-labeled DB-1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ-producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.Neutralization3,4:The LEAF™ purified antibody (Endotoxin in vivo andin vitro(Cat. No. 507808).Additional reported applications (for the relevant formats) include:Western blotting1, and immunohistochemistry2of paraformaldehyde-fixed, saponin-treated frozen tissue sections.
Host
Mouse
Reactivity
Mouse, Rat
Applications
ICFC - Quality tested
Conjugation
PE anti-rat IFN-gamma Antibody anti-IFN-gamma - DB-1, BioLegend, 507806
ELISA Capture1or ELISPOT Capture2:The purified DB-1 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated poly5109 antibody (Cat. No. 510901) as the detecting antibody and recombinant IFN-? (Cat. No. 565701) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture.Flow Cytometry5:The fluorochrome-labeled DB-1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-?-producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.Neutralization3,4:The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of rat IFN-? bioactivityin vivoandin vitro(Cat. No. 507808).Additional reported applications (for the relevant formats) include:Western blotting1, and immunohistochemistry2of paraformaldehyde-fixed, saponin-treated frozen tissue sections.
Host
Mouse
Reactivity
Mouse, Rat
Applications
ICFC - Quality tested
Conjugation
Purified anti-human IFN-gamma Antibody anti-IFN-gamma - MD-1, BioLegend, 507501
ELISA1,2,5and ELISPOT3,4Capture:The Purified MD-1 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4S.B3 (Cat. No. 502504/502514) antibody as the detecting antibody and recombinant human IFN-γ (Cat. No. 570209) as the standard. The LEAF™ Purified antibody is suggested for ELISPOT capture. For ELISPOT capture applications, a concentration range of 0.5-2 µg/ml is recommended.Neutralization1:The LEAF™ Purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IFN-γ bioactivity (Cat. No. 507513).Additional reported applications (for the relevant formats) include:Western blotting1,2, intracellular staining for flow cytometry6, and immunohistochemical staining7of paraformaldehyde-fixed, saponin-treated frozen tissue sections.Note:For testing human IFN-γ in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430101 to 430106) are specially developed and recommended.
Host
Mouse
Reactivity
Human
Applications
ELISA Capture -Quality testedICFC, IHC-F, WB -Reported in the literature, not verified in house
Conjugation
Purified anti-human IFN-gamma Antibody anti-IFN-gamma - MD-1, BioLegend, 507502
ELISA1,2,5and ELISPOT3,4Capture:The Purified MD-1 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4S.B3 (Cat. No. 502504/502514) antibody as the detecting antibody and recombinant human IFN-γ (Cat. No. 570209) as the standard. The LEAF™ Purified antibody is suggested for ELISPOT capture. For ELISPOT capture applications, a concentration range of 0.5-2 µg/ml is recommended.Neutralization1:The LEAF™ Purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IFN-γ bioactivity (Cat. No. 507513).Additional reported applications (for the relevant formats) include:Western blotting1,2, intracellular staining for flow cytometry6, and immunohistochemical staining7of paraformaldehyde-fixed, saponin-treated frozen tissue sections.Note:For testing human IFN-γ in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430101 to 430106) are specially developed and recommended.
Host
Mouse
Reactivity
Human
Applications
ELISA Capture -Quality testedICFC, IHC-F, WB -Reported in the literature, not verified in house
Conjugation
Pacific Blue anti-human IFN-gamma Antibody anti-IFN-gamma - 4S.B3, BioLegend, 502521
ELISA or ELISPOT Detection5:The biotinylated 4S.B3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified NIB42 antibody (Cat. No. 502402/502404) or purified MD-1 antibody (Cat. No. 507502/507513) as the capture antibody.Flow Cytometry3,4,6-8:The fluorochrome-labeled 4S.B3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ -producing cells within mixed cell populations.Additional reported applications (for the relevant formats) include:neutralization1,2, Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated tissue sections, and immunocytochemistry. The 4S.B3 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.Note:For testing human IFN-γ in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430101 to 430106) are specially developed and recommended.
Host
Mouse
Reactivity
Human
Applications
ICFC - Quality tested
Conjugation
Pacific Blue anti-human IFN-gamma Antibody anti-IFN-gamma - 4S.B3, BioLegend, 502522
ELISA or ELISPOT Detection5:The biotinylated 4S.B3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified NIB42 antibody (Cat. No. 502402/502404) or purified MD-1 antibody (Cat. No. 507502/507513) as the capture antibody.Flow Cytometry3,4,6-8:The fluorochrome-labeled 4S.B3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ -producing cells within mixed cell populations.Additional reported applications (for the relevant formats) include:neutralization1,2, Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated tissue sections, and immunocytochemistry. The 4S.B3 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.Note:For testing human IFN-γ in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430101 to 430106) are specially developed and recommended.
Host
Mouse
Reactivity
Human
Applications
ICFC - Quality tested
Conjugation
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