Search results for H2A.X
Histone H2A.X (H2AFX) Antibody, Abbexa, abx125925
H2AFX Antibody is a Rabbit Polyclonal against H2AFX.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Applications
ELISA, WB, IHC, IF/ICC
Conjugation
Unconjugated
Histone H2A.X (H2AX) Antibody, Abbexa, abx123069
Histone H2A.X (H2AX) Antibody is a Rabbit Polyclonal Antibody against Histone H2A.X (H2AX).
Host
Rabbit
Reactivity
Human, Mouse, Rat
Applications
IF/ICC
Conjugation
Unconjugated
Histone H2A.X (H2AFX) Antibody, Abbexa, abx027558
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
Host
Rabbit
Reactivity
Human
Applications
ELISA, WB, IHC
Conjugation
Unconjugated
Histone H2A.X (H2AFX) Antibody, Abbexa, abx034795
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Host
Mouse
Reactivity
Human
Applications
ELISA, WB, IHC
Conjugation
Unconjugated
Histone H2A.x (pS139) Antibody, Abbexa, abx000167
Histone H2A.x (pS139) Antibody is a Rabbit Polyclonal antibody against Histone H2A.x (pS139). Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Applications
ELISA, WB, IHC, IF/ICC
Conjugation
Unconjugated
Histone H2A.X (H2AFX) Antibody, Abbexa, abx320469
H2AFX Antibody is a Rabbit Polyclonal against H2AFX.
Host
Rabbit
Reactivity
Human
Applications
ELISA, IHC
Conjugation
Unconjugated
Histone H2A.X (H2AFX) Antibody, Abbexa, abx125927
H2AFX Antibody is a Rabbit Polyclonal against H2AFX.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Applications
ELISA, WB, IHC
Conjugation
Unconjugated
APC/Fire 750 anti-H2A.X Phospho Ser139 Antibody anti-H2A.X - 2F3, BioLegend, 613421
Additional reported applications (for the relevant formats of this clone) include:immunohistochemistry on paraffin embedded sections2, immunofluorescence microscopy3-9, Western blotting10-12, and flow cytometry1,13. Clone 2F3 cross-reacts with mouse4.Intracellular staining protocol for Anti-H2A.X-Phosphorylated (Ser139) Antibodyfor Flow Cytometry1. Prepare 70% absolute ethanol. Chill solution by storing at -20°C.2. Prepare cells of interest.3. Wash 1X with PBS, centrifuge at 350g for 5 min.4. Discard the supernatant and vortex to loosen cell pellet.5. Add pre-cooled 70% ethanol drop by drop, while vortexing.6. Incubate at -20°C for 60 minutes.7. Wash 3X withBioLegend Cell Staining Bufferand resuspend the cells at 0.5-1 X 107cells/ml in the cell staining buffer.8. Perform immunofluorescent staining for flow cytometry.
Host
Mouse
Reactivity
Human, Mouse
Applications
ICFC -Quality tested
Conjugation
APC/Fire 750 anti-H2A.X Phospho Ser139 Antibody anti-H2A.X - 2F3, BioLegend, 613422
Additional reported applications (for the relevant formats of this clone) include:immunohistochemistry on paraffin embedded sections2, immunofluorescence microscopy3-9, Western blotting10-12, and flow cytometry1,13. Clone 2F3 cross-reacts with mouse4.Intracellular staining protocol for Anti-H2A.X-Phosphorylated (Ser139) Antibodyfor Flow Cytometry1. Prepare 70% absolute ethanol. Chill solution by storing at -20°C.2. Prepare cells of interest.3. Wash 1X with PBS, centrifuge at 350g for 5 min.4. Discard the supernatant and vortex to loosen cell pellet.5. Add pre-cooled 70% ethanol drop by drop, while vortexing.6. Incubate at -20°C for 60 minutes.7. Wash 3X withBioLegend Cell Staining Bufferand resuspend the cells at 0.5-1 X 107cells/ml in the cell staining buffer.8. Perform immunofluorescent staining for flow cytometry.
Host
Mouse
Reactivity
Human, Mouse
Applications
ICFC -Quality tested
Conjugation
PerCP/Cyanine5.5 anti-H2A.X-Phosphorylated Ser139 Antibody anti-Phosphorylated H2A.X - 2F3, BioLegend, 613413
Additional reported applications (for the relevant formats of this clone) include:immunohistochemistry on paraffin embedded sections2, immunofluorescence microscopy3-9, Western blotting10-12, and flow cytometry1,13. Clone 2F3 cross-reacts with mouse4.Intracellular staining protocol for Anti-H2A.X-Phosphorylated (Ser139) Antibodyfor Flow Cytometry1. Prepare 70% absolute ethanol. Chill solution by storing at -20°C.2. Prepare cells of interest.3. Wash 1X with PBS, centrifuge at 350g for 5 min.4. Discard the supernatant and vortex to loosen cell pellet.5. Add pre-cooled 70% ethanol drop by drop, while vortexing.6. Incubate at -20°C for 60 minutes.7. Wash 3X withBioLegend Cell Staining Bufferand resuspend the cells at 0.5-1 X 107cells/ml in the cell staining buffer.8. Perform immunofluorescent staining for flow cytometry.
Host
Mouse
Reactivity
Human, Mouse
Applications
ICFC - Quality tested
Conjugation
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