Search results for 718
Spark Red™ 718 anti-human CD8a Antibody, CD8a, S21010F, BioLegend, 388805
Clone S21010F can completely block the binding of clone HIT8a, clone RPA-T8, and clone SK1 on target cells and can be blocked by HIT8a and SK1.Clone S21010F does not interfere with the binding of anti-human CD8β antibodies S21011A or QA20A40.Clone S21010F shows comparable staining signals on 4% paraformaldehyde-fixed cells and unfixed cells.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Spark Red™ 718 anti-human CD8a Antibody, CD8a, S21010F, BioLegend, 388806
Clone S21010F can completely block the binding of clone HIT8a, clone RPA-T8, and clone SK1 on target cells and can be blocked by HIT8a and SK1.Clone S21010F does not interfere with the binding of anti-human CD8β antibodies S21011A or QA20A40.Clone S21010F shows comparable staining signals on 4% paraformaldehyde-fixed cells and unfixed cells.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Spark Red™ 718 anti-human CD3 Antibody, CD3, OKT3, BioLegend, 317361
The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex.Clone OKT3 can block the binding of clones SK7 and UCHT1.4The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Spark Red™ 718 anti-human CD3 Antibody, CD3, OKT3, BioLegend, 317362
The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex.Clone OKT3 can block the binding of clones SK7 and UCHT1.4The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Spark Red™ 718 anti-mouse CD3 Antibody, CD3, 17A2, BioLegend, 100281
Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
Spark Red™ 718 anti-mouse CD3 Antibody, CD3, 17A2, BioLegend, 100282
Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.
Host
Rat
Reactivity
Mouse
Applications
FC - Quality tested
Conjugation
Spark Red™ 718 anti-human CD45 Antibody, CD45, 2D1, BioLegend, 368561
It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Spark Red™ 718 anti-human CD45 Antibody, CD45, 2D1, BioLegend, 368562
It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.
Host
Mouse
Reactivity
Human
Applications
FC - Quality tested
Conjugation
Spark Red™ 718 anti-human CD11c Antibody, CD11c, 3.9, BioLegend, 301649
Clone 3.9 preferentially binds the activated form of CD11c, is specific for the I domain of CD11c, and is able to partially block the binding of CD11c and ICAM-4. 3.9 binding is divalent cation dependent12. While analyzing blood, it is best to use heparin as the anti-coagulant and not EDTA. Since the ability of clone 3.9 to bind to its target is divalent cation dependent, the usage of EDTA as an anti-coagulant may be detrimental to staining due to its chelating properties.Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections4, and functional assays5,6. The LEAF™purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 301616). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 301632) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Applications
FC - Quality tested
Conjugation
Spark Red™ 718 anti-human CD11c Antibody, CD11c, 3.9, BioLegend, 301650
Clone 3.9 preferentially binds the activated form of CD11c, is specific for the I domain of CD11c, and is able to partially block the binding of CD11c and ICAM-4. 3.9 binding is divalent cation dependent12. While analyzing blood, it is best to use heparin as the anti-coagulant and not EDTA. Since the ability of clone 3.9 to bind to its target is divalent cation dependent, the usage of EDTA as an anti-coagulant may be detrimental to staining due to its chelating properties.Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections4, and functional assays5,6. The LEAF™purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 301616). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 301632) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Applications
FC - Quality tested
Conjugation
Please provide the required information below so that we can quickly source your products.