Western Blot Antibodies are antibodies for immunoblotting applications, capable of recognizing linear epitopes under denaturing conditions to enable qualitative and semi-quantitative analysis of target proteins. Key experimental steps include blocking (5% non-fat milk or BSA to minimize non-specific binding), antibody dilution (primary antibody typically 1:1000–1:10000 for optimal signal-to-noise ratio), thorough washing (TBST to remove unbound antibodies), matched secondary antibody (HRP- or fluorophore-conjugated, species-specific), and loading control normalization (β-actin, GAPDH, etc.). Primary applications encompass protein expression analysis (validation of overexpression or knockdown efficiency), post-translational modification detection (phosphorylation, acetylation status), protein-protein interaction verification (detection of co-immunoprecipitated proteins), biomarker screening (differential expression of disease-associated proteins), and antibody quality control, serving as fundamental tools in molecular biology and protein research.
Protein Tyrosine Phosphatase, Non Receptor Type 1 (PTPN1) Antibody is a Rabbit Polyclonal antibody against Protein Tyrosine Phosphatase, Non Receptor Type 1 (PTPN1).
The MLLT7 (Mixed-lineage leukemia translocated to 7) protein is a forkhead transcription factor that activates apoptosis by inducing the BCL-6 transcriptional repressor. MLL-MLLT7 fusion protein transformw myeloid progenitors and impairs forkhead protein function. MLLT7 is a target of the phosphatidylinositol 3-kinase/PKB insulin signaling pathway and the AMP-activated protein kinase-dependent pathway.
This product is currently in development. The lead time for this product may be several months. Please contact us at [email protected] for an updated lead time before purchasing this product. Glutamate Decarboxylase 2 (GAD2) Antibody is a Mouse Monoclonal antibody against Glutamate Decarboxylase 2 (GAD2). This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.
