Western Blot Antibodies are antibodies for immunoblotting applications, capable of recognizing linear epitopes under denaturing conditions to enable qualitative and semi-quantitative analysis of target proteins. Key experimental steps include blocking (5% non-fat milk or BSA to minimize non-specific binding), antibody dilution (primary antibody typically 1:1000–1:10000 for optimal signal-to-noise ratio), thorough washing (TBST to remove unbound antibodies), matched secondary antibody (HRP- or fluorophore-conjugated, species-specific), and loading control normalization (β-actin, GAPDH, etc.). Primary applications encompass protein expression analysis (validation of overexpression or knockdown efficiency), post-translational modification detection (phosphorylation, acetylation status), protein-protein interaction verification (detection of co-immunoprecipitated proteins), biomarker screening (differential expression of disease-associated proteins), and antibody quality control, serving as fundamental tools in molecular biology and protein research.
IMPA1 Antibody is a Rabbit Polyclonal antibody against IMPA1. This gene encodes an enyzme that dephosphorylates myo-inositol monophosphate to generate free myo-inositol, a precursor of phosphatidylinositol, and is therefore an important modulator of intracellular signal transduction via the production of the second messengers myoinositol 1,4,5-trisphosphate and diacylglycerol. This enzyme can also use myo-inositol-1,3-diphosphate, myo-inositol-1,4-diphosphate, scyllo-inositol-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates. This enzyme shows magnesium-dependent phosphatase activity and is inhibited by therapeutic concentrations of lithium. Inhibition of inositol monophosphate hydroylosis and subsequent depletion of inositol for phosphatidylinositol synthesis may explain the anti-manic and anti-depressive effects of lithium administered to treat bipolar disorder. Alternative splicing results in multiple transcript variants encoding distinct isoforms. A pseudogene of this gene is also present on chromosome 8q21.13.
