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Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit Monoclonal Antibody detects endogenous levels of p44 and p42 MAP Kinase (Erk1 and Erk2) when dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), and singly phosphorylated at Tyr204. This antibody does not cross-react with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinase.
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey, Mink, D. melanogaster, Zebrafish Antibodies, Pig
Applications
Western Blotting: 1:1000 Fluorescent Western: 1:1000 Simple Western<sup>TM</sup>: 1:10 - 1:50 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400
Conjugation
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Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (20G11) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of p44 and p42 MAP Kinase (Erk1 and Erk2) when dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), and singly phosphorylated at Thr202. This antibody does not cross-react with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinase.
Host
Rabbit
Reactivity
Human, Mouse, Rat, Hamster, Monkey, Mink, D. melanogaster, Zebrafish Antibodies, Pig, S. cerevisiae
Applications
Conjugation
.png)
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (20G11) Rabbit Monoclonal Antibody detects endogenous levels of p44 and p42 MAP Kinase (Erk1 and Erk2) when dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), and singly phosphorylated at Thr202. This antibody does not cross-react with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinase.
Host
Rabbit
Reactivity
Human, Mouse, Rat, Hamster, Monkey, Mink, D. melanogaster, Zebrafish Antibodies, Pig, S. cerevisiae
Applications
Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:400 - 1:1600
Conjugation
.png)
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody detects endogenous levels of p44 and p42 MAP Kinase (Erk1 and Erk2) when phosphorylated either individually or dually at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2). This antibody does not cross-react with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP Kinase, and does not cross-react with non-phosphorylated Erk1/2.
Host
Reactivity
Human, Mouse, Rat, Hamster, Monkey, Mink, D. melanogaster, Zebrafish Antibodies, Bovine, Pig, C. elegans
Applications
Western Blotting: 1:1000 Simple Western<sup>TM</sup>: 1:10 - 1:50 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400
Conjugation
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Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of p44 and p42 MAP Kinase (Erk1 and Erk2) when dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), and singly phosphorylated at Thr202. This antibody does not cross-react with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinases.
Host
Rabbit
Reactivity
Human, Mouse, Rat, Hamster, Monkey, Mink, D. melanogaster, Zebrafish Antibodies, Bovine, Dog, Pig, S. cerevisiae
Applications
Conjugation
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Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) Rabbit Monoclonal Antibody detects endogenous levels of p44 and p42 MAP Kinase (Erk1 and Erk2) when dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), and singly phosphorylated at Thr202. This antibody does not cross-react with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinases.
Host
Rabbit
Reactivity
Human, Mouse, Rat, Hamster, Monkey, Mink, D. melanogaster, Zebrafish Antibodies, Bovine, Dog, Pig, S. cerevisiae
Applications
Western Blotting: 1:2000 Fluorescent Western: 1:2000 Simple Western<sup>TM</sup>: 1:10 - 1:50 Immunoprecipitation: 1:50 IHC Leica Bond: 1:400 - 1:1600 Immunohistochemistry (Paraffin): 1:200 - 1:800 Immunofluorescence (Immunocytochemistry): 1:200 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600
Conjugation
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Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse Monoclonal Antibody detects endogenous levels of p44 and p42 MAP Kinase (Erk1 and Erk2) when dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), but not singly phosphorylated at Thr202 or Tyr204. This antibody does not cross-react with the corresponding phosphorylated residues of either SAPK/JNK or p38 MAP kinase. This antibody may cross-react with an unknown cytoskeletal protein in some cell lines as visualized by immunofluorescence.
Host
Mouse
Reactivity
Human, Mouse, Rat, Hamster, Monkey, Mink, Zebrafish Antibodies, Bovine, Pig
Applications
Western Blotting: 1:2000 Simple Western<sup>TM</sup>: 1:10 - 1:50 Flow Cytometry (Fixed/Permeabilized): 1:1000 - 1:2000
Conjugation
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Phospho-S6 Ribosomal Protein (Ser240/244) Antibody detects endogenous levels of ribosomal protein S6 only when phosphorylated at serines 240 and 244. This antibody does not detect S6 ribosomal protein phosphorylated at other sites.
Host
Reactivity
Human, Mouse, Rat, Monkey, Zebrafish Antibodies
Applications
Western Blotting: 1:1000 Immunoprecipitation: 1:50
Conjugation
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Histone H2A Antibody II detects endogenous levels of total histone H2A protein. This antibody also detects histone H2A.X and histone H2A.Z.
Host
Reactivity
Human, Mouse, Rat, Monkey, Zebrafish Antibodies
Applications
Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:100
Conjugation
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Histone H2A (D6O3A) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total histone H2A protein. This antibody does not cross-react with other histone proteins.
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey, Zebrafish Antibodies, Guinea Pig
Applications
Conjugation
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