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Phospho-Akt (Ser473) (D9E) Rabbit Monoclonal Antibody (mFluorTM Violet 610 Conjugate) detects endogenous levels of Akt1 only when phosphorylated at Ser473. This antibody conjugate also recognizes Akt2 and Akt3 when phosphorylated at the corresponding residues.
Host
Rabbit
Reactivity
Human, Mouse, Rat, Hamster, Monkey, D. melanogaster, Zebrafish Antibodies, Bovine
Applications
Flow Cytometry (Fixed/Permeabilized): 1:50
Conjugation
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Phospho-Akt (Ser473) (D9E) Rabbit Monoclonal Antibody (Pacific BlueTM Conjugate) detects endogenous levels of Akt protein only when phosphorylated at Ser473.
Host
Rabbit
Reactivity
Human, Mouse, Rat, Hamster, Monkey, D. melanogaster, Zebrafish Antibodies, Bovine
Applications
Flow Cytometry (Fixed/Permeabilized): 1:50
Conjugation
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Phospho-Akt (Ser473) (D9E) Rabbit Monoclonal Antibody (PE Conjugate) detects endogenous levels of Akt only when phosphorylated at Ser473.
Host
Rabbit
Reactivity
Human, Mouse, Rat, Hamster, Monkey, D. melanogaster, Zebrafish Antibodies, Bovine
Applications
Flow Cytometry (Fixed/Permeabilized): 1:50
Conjugation
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Phospho-Akt (Ser473) (D9E) Rabbit Monoclonal Antibody (PE-Cy7®Conjugate) detects endogenous levels of Akt only when phosphorylated at Ser473.
Host
Rabbit
Reactivity
Human, Mouse, Rat, Hamster, Monkey, D. melanogaster, Zebrafish Antibodies, Bovine
Applications
Flow Cytometry (Fixed/Permeabilized): 1:50
Conjugation
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beta-Actin Antibody detects endogenous levels of β-actin. Due to the high sequence identity between the actin isoforms, this antibody may cross-react with other actin isoforms.
Host
Reactivity
Human, Mouse, Rat, Hamster, Monkey, Mink, D. melanogaster, Zebrafish Antibodies, Bovine
Applications
Western Blotting: 1:1000
Conjugation
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beta-Actin (D6A8) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total β-actin protein. Due to the high sequence identity between the cytoplasmic actin isoforms, β-actin and cytoplasmic γ-actin, this antibody may cross-react with cytoplasmic γ-actin. This antibody does not cross-react with α-skeletal, α-cardiac, α-vascular smooth, or γ-enteric smooth muscle isoforms.
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey, D. melanogaster, Zebrafish Antibodies
Applications
Conjugation
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beta-Actin (D6A8) Rabbit Monoclonal Antibody recognizes endogenous levels of total β-actin protein. Due to the high sequence identity between the cytoplasmic actin isoforms, β-actin and cytoplasmic γ-actin, this antibody may cross-react with cytoplasmic γ-actin. This antibody does not cross-react with α-skeletal, α-cardiac, α-vascular smooth, or γ-enteric smooth muscle isoforms.
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey, D. melanogaster, Zebrafish Antibodies
Applications
Western Blotting: 1:1000 Fluorescent Western: 1:1000 Immunofluorescence (Immunocytochemistry): 1:100 - 1:200 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200
Conjugation
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beta-Tubulin (9F3) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total β-tubulin protein, and does not cross-react with recombinant α-tubulin.
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey, Zebrafish Antibodies, Bovine
Applications
Conjugation
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beta-Tubulin (9F3) Rabbit Monoclonal Antibody detects endogenous levels of total β-tubulin protein, and does not cross-react with recombinant α-tubulin.
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey, Zebrafish Antibodies, Bovine
Applications
Western Blotting: 1:1000 Fluorescent Western: 1:1000 Immunohistochemistry (Paraffin): 1:50 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200
Conjugation
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The beta-Tubulin Antibody detects endogenous levels of total β-tubulin protein, and does not cross-react with recombinant α-tubulin.
Host
Reactivity
Human, Mouse, Rat, Monkey, Zebrafish Antibodies, Bovine
Applications
Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:75 - 1:300 Immunofluorescence (Immunocytochemistry): 1:50 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200
Conjugation
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