Isotype Controls

Rat IgG1 Isotype Control Antibody, InvivoGen, mabg1-ctlrt

Rat IgG2a Isotype Control Antibody, InvivoGen, mabg2a-ctlrt

Recombinant Mouse IgG1 Isotype Control Antibody, InvivoGen, bgal-mab9-1

InvivoGen provides arecombinantanti-β-Gal-mIgG1 monoclonal antibody (mAb)  that was previously extracted from hybridoma. It is now expressed and produced in chinese hamster ovary (CHO) cells, ensuring reliability and lot-to-lot reproducibility. Thereby, common hybridoma-related drawbacks such as the generation of non-relevant mAbs containing aberrant light chains are avoided. Thiscontrol antibodytargetsE. coli'sβ-galactosidase (β-Gal), an antigen that is not present in mice. In addition, it displayslow ADCC(antibody-dependent cell-mediated cytotoxicity) andno CDC(complement-dependent cytotoxicity) effector functions [1]. Thesequenceof Anti-β-Gal-mIgG1 is100% murine(constant and variable regions), as the original clone (clone T9C6) was raised in mice using a proprietary method. This feature allows for reduced immunogenicity and risks of fatal hypersensitivity reactions upon repeated mAb injections into mice [2].

Recombinant Mouse IgG1e3 Isotype Control Antibody - Fc silent (D265A), InvivoGen, bgal-mab15-1

InvivoGen provides arecombinantanti-β-Gal-mIgG1e3 monoclonal antibody (mAb) that was previously extracted from hybridoma. It is now expressed and produced in Chinese hamster ovary (CHO) cells, ensuring reliability and lot-to-lot reproducibility. Thereby, common hybridoma-related drawbacks such as the generation of non-relevant mAbs containing aberrant light chains are avoided [1]. Thiscontrol antibodytargetsE. coli'sβ-galactosidase (β-Gal), an irrelevant antigen that is not present in mice. Thesequenceof Anti-β-Gal-mIgG1e3 is100% murine(constant and variable regions), as the original clone (clone T9C6) was raised in mice using a proprietary method. Within the engineered murine IgG1e3 isotype, a point mutation D265A was introduced leading toa complete lossof unwanted Fc-associatedeffector functions[2]. Altogether, these alterations increase antibody performance and overcome immunogenic events, especially upon repeated mAb injections into mice [3].

Recombinant Mouse IgG2a Isotype Control Antibody, InvivoGen, bgal-mab10-1

InvivoGen provides arecombinantanti-β-Gal-mIgG2a monoclonal antibody (mAb). It is expressed and produced in chinese hamster ovary (CHO) cells, ensuring reliability and lot-to-lot reproducibility. Thereby, common hybridoma-related drawbacks such as the generation of non-relevant mAbs containing aberrant light chains are avoided. Thiscontrol antibodytargetsE. coli'sβ-galactosidase (β-Gal), an antigen that is not present in mice. In addition, it displaysvery high ADCP(antibody-dependent cell-mediated phagocytosis),high ADCC(antibody-dependent cell-mediated cytotoxicity) andhigh CDC(complement-dependent cytotoxicity) effector functions [1]. Thesequenceof Anti-β-Gal-mIgG2a InvivoFit^TM is100% murine(constant and variable regions), as the original clone (clone T9C6) was raised in mice using a proprietary method. This feature allows for reduced immunogenicity and risks of fatal hypersensitivity reactions upon repeated mAb injections into mice [2].

Recombinant Mouse IgG2a Isotype Control Antibody - Fc silent (LALA-PG), InvivoGen, bgal-mab19-1

The recombinantAnti-β-Gal-mIgG2ae3is an Fc silent monoclonal antibody (mAb) that serve as a negative control antibodyin vivo. This control antibody targetsE. coli's β-galactosidase (β-Gal), an antigen that is not present in mice. Its engineered mouse IgG2a isotype contains three point mutations in the Fc region at positionsL234A, L235A, andP329G. These mutations, also referred to as LALA-PG, result in the complete loss of unwanted Fc-associated effector functions. Anti-β-Gal-mIgG2ae3 is expressed and produced in Chinese hamster ovary (CHO) cells, ensuring reliability and lot-to-lot reproducibility. Thereby, common hybridoma-related drawbacks such as the generation of non-relevant mAbs containing aberrant light chains are avoided. Due to the triple mutation LALA-PG, it displaysno ADCP(antibody-dependent cell-mediated phagocytosis),no ADCC(antibody-dependent cell-mediated cytotoxicity) andno CDC(complement-dependent cytotoxicity) effector functions. Thesequenceof Anti-β-Gal-mIgG2ae3 InvivoFit^TM is100% murine(constant and variable regions), as the original clone (clone T9C6) was raised in mice using a proprietary method. This feature allows for reduced immunogenicity and risks of fatal hypersensitivity reactions upon repeated mAb injections into mice [2].

Human IgG4 (S228P/L235E/D265A/R409K), kappa Isotype Control Antibody (HyHEL-10) (HV799083), abinScience, HV799083

Human IgG1 (M428L/N434S), kappa Isotype Control Antibody (13R4) (HV080293), abinScience, HV080293

Human IgG1 (K97R) Isotype Control Antibody (HyHEL-10) (HP558107), abinScience, HP558107

Human IgG2 Isotype Control Antibody (HyHEL-10) (HV988107), abinScience, HV988107